Search

Figure S3. 1,25-(OH)2VitaminD3, does not affect Pin1 stability and function. a, b The expression of CD11b were assayed by FACS in HL-60, U937 and KG-1a at 72Â h after treatment (a). 1, 25-D3 induces HL-60 and U937 differentiation, but not KG-1a. The differentiation state of each cell was assayed by the percentages of CD11b positive cells in indicated cell lines (b). c Pin1 protein levels were not changed after 72Â h incubation of 1, 25-D3 in HL-60 and U937. d 1,25-D3 does not inhibit PPIase activity of Pin1. Pin1 was incubated with different concentrations of 1, 25-D3, followed by chymotrypsin-coupled PPIase assay. e Pin1 downstream oncoproteins were assayed after 72Â h incubation of 1,25-D3 in U937. (PDF 2712 kb)

Figure S2. The relative intensities of Pin1 downstream oncoproteins in Vec and shPin1. The intensities of each protein signal were determined by ImageJ. The semi-quantitative results were averaged from three independent experiments. (PDF 312 kb)

Figure S4. Immortalized normal blood cells were resistant to ATRA. a Pin1 protein levels in two immortalized normal blood cells (N1 and N5 cells) were assayed by immunoblotting and compared with AML cell lines (HL-60, U937 and KG-1a). N was indicated normal blood cells. b After 3Â days treatment of different concentrations of ATRA, cell growth rates were determined by CellTiter-GloÂŽ 2.0 Assay. N1 and N5 cells were completely resistant to ATRA, compared with leukemia cell lines. (PDF 222 kb)

Figure S4. Immortalized normal blood cells were resistant to ATRA. a Pin1 protein levels in two immortalized normal blood cells (N1 and N5 cells) were assayed by immunoblotting and compared with AML cell lines (HL-60, U937 and KG-1a). N was indicated normal blood cells. b After 3Â days treatment of different concentrations of ATRA, cell growth rates were determined by CellTiter-GloÂŽ 2.0 Assay. N1 and N5 cells were completely resistant to ATRA, compared with leukemia cell lines. (PDF 222 kb)

Figure S1. The original whole blots of Pin1 expression in AML patients and leukemia cell lines. a The stronger signals of Pin1 protein levels of healthy controls and AML patients were detected with a 5 min exposure of blot than with a 30 s exposure of blot. b The stronger signals of Pin1 protein levels of healthy controls and leukemia cell lines were detected with a 5 min exposure of blot than with a 30 s exposure of blot. (PDF 1205 kb)

Figure S2. The relative intensities of Pin1 downstream oncoproteins in Vec and shPin1. The intensities of each protein signal were determined by ImageJ. The semi-quantitative results were averaged from three independent experiments. (PDF 312 kb)

Figure S1. The original whole blots of Pin1 expression in AML patients and leukemia cell lines. a The stronger signals of Pin1 protein levels of healthy controls and AML patients were detected with a 5 min exposure of blot than with a 30 s exposure of blot. b The stronger signals of Pin1 protein levels of healthy controls and leukemia cell lines were detected with a 5 min exposure of blot than with a 30 s exposure of blot. (PDF 1205 kb)