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Additional file 5: Figure S5. YAP can reverse the inhibitory effect of LOXL1. Transwell migration and Matrigel invasion assays were performed in LOXL1 alone or LOXL1 and YAP co-transfected HCT8 cells. Representative images (left panel) and quantification (right panel) are shown as indicated. Data from three independent experiments are presented as the mean ± SD. Statistical significance was assessed by unpaired t-test; **P

Additional file 2: Figure S2. Knockdown of LOXL1 in RKO and HT29 cells increases their proliferation ability in vitro. CCK8 analysis was performed to detect the cell proliferation. Data from three independent experiments are presented as the mean ± SD. Statistical significance was assessed by unpaired t-test; ***P

Additional file 1: Figure S1. LOXL1 overexpression inhibits the proliferation of CRC cells. a HCT8-N/HCT8-LOXL1 and SW480-N/SW480-LOXL1 cells were detected by CCK8 analysis. Data are shown as the mean ± SD of triplicate independent sets of experiments; statistical significance was assessed by unpaired t-test. ***P

Additional file 3: Figure S3. LOXL1 negatively regulates the YAP activity. a LOXL1 and YAP constructs alone or combined, as indicated, were transfected into HEK293T cells together with a indicated luciferase reporters. The results shown were normalized for transfection efficiency. Data are shown as the mean ± SD of triplicate independent sets of experiments; statistical significance was assessed by unpaired t-test. ns; non-significant, ***P

Additional file 6: Figure S6. a H-E staining of HCT8-N/HCT8-LOXL1 and SW480-N/SW480-LOXL1 xenograft tumours. b and c HCT8-N/HCT8-LOXL1 and SW480-N/SW480-LOXL1 xenograft tumours were detected the p-YAP(S127) and Ki67 levels by immunohistochemistry. d Cell apoptosis in xenografted tumors was measured by the TUNEL assay. Scale bar: 100 μm.

Additional file 4: Figure S4 Knockdown of LOXL1 decreases the Hippo pathway activation. HT29 cells were transfected with siRNA to LOXL1 or a control siRNA (N) for 48 h. Cell lysates were analysed by immunoblotting with the indicated antibodies.