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Additional file 9: Supplementary Table 3. Several suppressor microRNAs whose levels decreased under KITENIN overexpression but recovered after DKC1125. Suppressor miRNAs whose levels were decreased to a greater extent in CRC cells expressing high KITENIN than in parental cells (> 25%) and restored after treatment with DKC1125 (Supplementary Table 1 and 2) are summarized. Data were represented as mean ± SEM (n = 3). In addition to miR-124-3p, miR-143-3p, and miR-150-5p, several other suppressor miRNAs are shown: miR-100-5p, miR-125a-5p, miR-125b-5p, miR-140-5p, miR-218-5p, miR-34c-5p, miR-98-5p, miR-101-3p, miR-133a-3p, miR-216b-5p, miR-34b-3p, miR-486-5p, miR-502-5p, and miR-622.

Additional file 7: Supplementary Table 1. Changes in microRNAs associated with the cancer pathway under KITENIN overexpression and the effects of DKC1125 on these changes. The expression of microRNAs were examined using the Pathway-focused miScript miRNA PCR array. Data were represented as mean ± SEM (n = 3). C, empty vector-transfected CRC cells in control group; CK, CRC cells overexpressing KITENIN in control group; C-V, empty vector-transfected CRC cells treated with vehicle (0.1% DMSO) alone; CK-V, CRC cells overexpressing KITENIN treated with vehicle alone; C-DKC, empty vector-transfected CRC cells treated with DKC1125 (0.5 μM); CK-DKC, CRC cells overexpressing KITENIN treated with DKC1125.

Additional file 8: Supplementary Table 2. Changes in tumor suppressor microRNAs under KITENIN overexpression, and the effects of DKC1125 on these changes. Based on the results of suppressor miRNA in KITENIN-overexpressed CRC cells (Supplementary Table 1), suppressor miRNAs whose levels changed in CRC cells expressing high KITENIN and following treatment with DKC1125 were investigated using the Pathway-focused miScript miRNA PCR array. Data were represented as mean ± SEM (n = 3).

Herbal information of five components of GJD used in this study. (DOCX 15Â kb)