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Tet2 loss leads to increased hematopoietic stem cell in mouse bone marrow. (a) The representative data of FACS analysis of wild-type and Tet2 knockout HSCs. The cells were stained with antibodies to lineage, Sca1, and c-Kit markers. The lineage negative population was gated first. Numbers indicate percent cells within Lin-c-Kit+Sca1+ gates. (b) The representative FACS data of GFP+ population from wild-type and Tet2−/− mouse. The lineage, Sca1, and c-Kit markers were stained and gated first. The GFP+ population from Lin-Sca1+c-Kit+ was compared between wild-type and Tet2−/− mouse. (PDF 642 kb)

The fluorescence intensity of dividing Tet2–/– and Tet2−/−;Flt3ITD dividing HSCs. (a) The GFP pixel intensity unit (PIU) of Tet2–/– dividing HSCs was compared with wild-type control. (b) The GFP PIU of Tet2–/–;Flt3ITD dividing HSCs was compared with wild-type control. (PDF 552 kb)

OP9 stromal cells contribute to the fate decision of precursors ex vivo. (a) The frequency of symmetric self-renewal, symmetric differentiation, and asymmetric division of GFP+ precursors from wild-type mouse treated with OP9 supernatant during 24Â h is shown. The data are a summary from three independent experiments. Error bars represent standard error of the mean (SEM). (b) The frequency of symmetric self-renewal, symmetric differentiation, and asymmetric division of GFP+ precursors from wild-type mouse seeded on irradiated OP9 cells during 24Â h is shown. The data are a summary from three independent experiments. Error bars represent SEM. (PDF 420Â kb)