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Additional file 2: Table S2: Primer sequences for RT-PCR.

Additional file 3: Table S3: Clinicopathological parameters of CC patients in the CC cohort for IHC.

Additional file 6: Figure S1: Flow chart of the study. Five public colon cancer-related datasets were chosen for this study. First, we obtained CYT-related DEGs. Next, a two-gene prognostic model associated with CYT was established and validated. Finally, we verified the correlation between the model and T-cell infiltration. Figure S2: a-b LASSO Cox analysis identified the two genes most correlated with overall survival (OS). c-d Effect of HOXC8 and MS4A2 expression on the OS of CC patients in the whole TCGA cohort. Figure S3: Kaplan-Meier survival, risk score and time-dependent receiver operating characteristic (ROC) curves of the model for the testing cohort a-c and whole cohort d-f in CC. a, d Overall survival (OS) was significantly higher in the low-risk-score group than in the high-risk-score group. b, e The relationship between the risk score (upper panel) and expression of two prognostic immune genes (lower panel) is shown. c, f Time-dependent ROC curve analysis. Relationship between the expression of the risk score and g the TNM stage, h T stage, i M stage and j N stage in the IHC cohort.

Additional file 1: Table S1: Clinical features of CC patients in training set and testing set.

Additional file 4: Table S4: The univariate Cox HR regression result of selected genes.

Additional file 5: Table S5: The specific statistical parameters of HOXC8 and MS4A2 in each group.