13 results on '"Hong-Liang Zhang"'
Search Results
2. Minor envelope proteins from GP2a to GP4 contribute to the spread pattern and yield of type 2 PRRSV in MARC-145 cells
- Author
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Yuan-Zhe Bai, Yue Sun, Yong-Gang Liu, Hong-Liang Zhang, Tong-Qing An, Qian Wang, Zhi-Jun Tian, Xinyuan Qiao, Xue-Hui Cai, and Yan-Dong Tang
- Subjects
spread pattern ,PRRSV ,cell-to-cell ,cell-free ,yield ,Microbiology ,QR1-502 - Abstract
In China, porcine reproductive and respiratory syndrome virus (PRRSV) vaccines are widely used. These vaccines, which contain inactivated and live attenuated vaccines (LAVs), are produced by MARC-145 cells derived from the monkey kidney cell line. However, some PRRSV strains in MARC-145 cells have a low yield. Here, we used two type 2 PRRSV strains (CH-1R and HuN4) to identify the genes responsible for virus yield in MARC-145 cells. Our findings indicate that the two viruses have different spread patterns, which ultimately determine their yield. By replacing the viral envelope genes with a reverse genetics system, we discovered that the minor envelope proteins, from GP2a to GP4, play a crucial role in determining the spread pattern and yield of type 2 PRRSV in MARC-145 cells. The cell-free transmission pattern of type 2 PRRSV appears to be more efficient than the cell-to-cell transmission pattern. Overall, these findings suggest that GP2a to GP4 contributes to the spread pattern and yield of type 2 PRRSV.
- Published
- 2024
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3. Is Guillain–Barré syndrome related to systemic lupus erythematosus or other autoimmune diseases?
- Author
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Linpei Jia, Fengming Ni, and Hong-Liang Zhang
- Subjects
Guillain-Barré syndrome ,systemic lupus erythematosus ,peripheral nervous system ,cerebrospinal fluid ,autoimmune disease ,Neurology. Diseases of the nervous system ,RC346-429 - Published
- 2024
- Full Text
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4. Caffeine intake improves the cognitive performance of patients with chronic kidney disease
- Author
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Linpei Jia, Hanxue Zhao, Lixiao Hao, Lin-Hui Jia, Rufu Jia, and Hong-Liang Zhang
- Subjects
caffeine ,chronic kidney disease ,cognitive performance ,the National Health and Nutrition Examination Survey ,cognitive impairment ,Medicine (General) ,R5-920 - Abstract
ObjectiveCognitive impairment is a common complication of chronic kidney disease (CKD). Caffeine intake has been reported to improve cognitive performance in several studies. However, whether the benefits of caffeine intake on cognitive function apply to patients with CKD remains unknown.MethodsWe performed a retrospective cross-sectional study based on the National Health and Nutrition Examination Survey (NHANES). The data of CKD subjects and non-CKD subjects from NHANES 2011−2014 were analyzed. Propensity score matching (PSM) was performed based on age, sex, diabetes, cancer, educational level, energy intake and protein intake to select subjects. The Consortium to Establish a Registry for Alzheimer’s Disease Word Learning Test (CERAD-WL), the CERAD Word List Recall Test (CERAD-DR), the Animal Fluency Test (AF) and the Digit Symbol Substitution Test (DSST) were used, whereby the occurrence of cognitive impairment was identified. Logistic regression models were performed to evaluate the association between caffeine intake and cognitive performance in CKD and non-CKD participants. Stratified analyses according to the stage of CKD and the urinary albumin/creatinine ratio levels were performed. Plot curves were then generalized to present a non-linear relationship, and the inflection point for each non-linear model was obtained by using a recursive algorithm.ResultsCognitive impairment was more prevalent in CKD patients than in non-CKD subjects. For CKD patients, caffeine intake was associated with higher CERAD-WL, CERAD-DR, AF and DSST scores. For non-CKD subjects, caffeine intake was associated with higher DSST scores only. Subgroup analysis revealed that caffeine only benefited the cognitive function of patients with CKD stages 2 and 3. The analysis showed non-linear relationships of caffeine intake and cognitive function for both CKD and non-CKD subjects. The inflection point of caffeine intake for CKD patients was 279 mg/day.ConclusionThe recommended dose of caffeine intake to improve the cognitive function of CKD patients is ≤279 mg/day.
- Published
- 2022
- Full Text
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5. Evasion of Antiviral Innate Immunity by Porcine Reproductive and Respiratory Syndrome Virus
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Tong-Yun Wang, Ming-Xia Sun, Hong-Liang Zhang, Gang Wang, Guoqing Zhan, Zhi-Jun Tian, Xue-Hui Cai, Chenhe Su, and Yan-Dong Tang
- Subjects
antiviral innate immunity ,PRRSV ,PRRs ,interferon ,JAK-STAT ,immune evasion ,Microbiology ,QR1-502 - Abstract
Innate immunity is the front line for antiviral immune responses and bridges adaptive immunity against viral infections. However, various viruses have evolved many strategies to evade host innate immunity. A typical virus is the porcine reproductive and respiratory syndrome virus (PRRSV), one of the most globally devastating viruses threatening the swine industry worldwide. PRRSV engages several strategies to evade the porcine innate immune responses. This review focus on the underlying mechanisms employed by PRRSV to evade pattern recognition receptors signaling pathways, type I interferon (IFN-α/β) receptor (IFNAR)-JAK-STAT signaling pathway, and interferon-stimulated genes. Deciphering the antiviral immune evasion mechanisms by PRRSV will enhance our understanding of PRRSV’s pathogenesis and help us to develop more effective methods to control and eliminate PRRSV.
- Published
- 2021
- Full Text
- View/download PDF
6. Intensive Care and Treatment of Severe Guillain–Barré Syndrome
- Author
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Pei Shang, Jiachun Feng, Wei Wu, and Hong-Liang Zhang
- Subjects
Guillain–Barré syndrome ,intensive care ,intravenous immunoglobulin ,mechanical ventilation ,plasma exchange ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Guillain–Barré syndrome (GBS) is an acute polyneuropathy mostly characterized by acute flaccid paralysis with or without sensory/autonomous nerve dysfunction. Current immuno therapies including intravenous immunoglobulin (IVIg), plasma exchange (PE), and newly developed biological drugs benefit patients by alleviating hyperreactive immune responses. Up to 30% of patients develop respiratory failure during hospitalization and require mechanical ventilation and intensive care. Immunotherapies, mechanical ventilation, supportive care, and complication management during the intensive care unit (ICU) stay are equally emphasized. The most important aspect of intensive care and treatment of severe GBS, that is, mechanical ventilation, has been extensively reviewed elsewhere. In contrast to immunotherapies, care and treatment of GBS in the ICU setting are largely empirical. In this review, we intend to stress the importance of intensive care and treatment, other than mechanical ventilation in patients with severe GBS. We summarize the up-to-date knowledge of pharmacological therapies and ICU management of patients with severe GBS. We aim to answer some key clinical questions related to the management of severe GBS patients including but not limited to: Is IVIg better than PE or vice versa? Whether combinations of immune therapies benefit more? How about the emerging therapies promising for GBS? When to perform tracheal intubation or tracheostomy? How to provide multidisciplinary supportive care for severe cases? How to avert life-threatening complications in severe cases?
- Published
- 2021
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7. Pharmacological Targeting of Microglial Activation: New Therapeutic Approach
- Author
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Cai-Yun Liu, Xu Wang, Chang Liu, and Hong-Liang Zhang
- Subjects
Parkinson’s disease ,microglia ,neuroinflammation ,microglial activation ,polarization ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Mounting evidence suggests that neuroinflammation is not just a consequence but a vital contributor to the development and progression of Parkinson’s disease (PD). Microglia in particular, may contribute to the induction and modulation of inflammation in PD. Upon stimulation, microglia convert into activated phenotypes, which exist along a dynamic continuum and bear different immune properties depending on the disease stage and severity. Activated microglia release various factors involved in neuroinflammation, such as cytokines, chemokines, growth factors, reactive oxygen species (ROS), reactive nitrogen species (RNS), and prostaglandins (PGs). Further, activated microglia interact with other cell types (e.g., neurons, astrocytes and mast cells) and are closely associated with α-synuclein (α-syn) pathophysiology and iron homeostasis disturbance. Taken together, microglial activation and microglia-mediated inflammatory responses play essential roles in the pathogenesis of PD and elucidation of the complexity and imbalance of microglial activation may shed light on novel therapeutic approaches for PD.
- Published
- 2019
- Full Text
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8. Blood Brain Barrier Permeability Could Be a Biomarker to Predict Severity of Neuromyelitis Optica Spectrum Disorders: A Retrospective Analysis
- Author
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Ying Wang, Mingqin Zhu, Caiyun Liu, Jinming Han, Wenjuan Lang, Yang Gao, Chao Lu, Shuang Wang, Shuai Hou, Nannan Zheng, Dong Wang, Yang Chen, Yu Zhang, Hong-Liang Zhang, and Jie Zhu
- Subjects
blood brain barrier ,neuromyelitis optica spectrum disorders ,aquaporin 4 ,albumin index ,biomarker ,Expanded Disability Status Scale ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Background: Blood-brain barrier (BBB) pathology exists in neuromyelitis optica spectrum disorders (NMOSD). However, the clinical use of BBB permeability, such as predicting disease severity of NMOSD, has rarely been studied in a large cohort of patients.Objectives: The current study explored the association between BBB permeability and clinical parameters in order to assess if BBB permeability could be a biomarker to predict disease severity and clinical characteristics of NMOSD.Methods: Among 69 enrolled NMOSD patients, 47 with albumin index over 5 × 10−3 were assigned to the increased BBB permeability group, and the remaining 22 were to the normal BBB permeability group. Disease severity was assessed using the Expanded Disability Status Scale (EDSS).Results: Patients in the increased BBB permeability group had significantly higher EDSS scores, anti-aquporin-4 immunoglobulin G titers, more dense cerebrospinal fluid protein concentrations, white blood cell counts, myelin basic protein levels and more dense complement 3 concentrations than found in the comparative normal BBB permeability group. The albumin index was positively correlated to the length of lesions in spinal cord.Conclusions: BBB permeability was associated with clinical features, laboratory results and radiological data of NMOSD patients, and may be a potential biomarker to predict disease severity and clinical characteristics of NMOSD.
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- 2018
- Full Text
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9. Differentiation of Glial Cells From hiPSCs: Potential Applications in Neurological Diseases and Cell Replacement Therapy
- Author
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Wei Zheng, Qian Li, Chao Zhao, Yuwei Da, Hong-Liang Zhang, and Zhiguo Chen
- Subjects
astrocytes ,oligodendrocytes ,microglia ,hiPSC ,differentiation ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Glial cells are the most abundant cell type in the central nervous system (CNS) and play essential roles in maintaining brain homeostasis, forming myelin, and providing support and protection for neurons, etc. Over the past decade, significant progress has been made in the reprogramming field. Given the limited accessibility of human glial cells, in vitro differentiation of human induced pluripotent stem cells (hiPSCs) into glia may provide not only a valuable research tool for a better understanding of the functions of glia in the CNS but also a potential cellular source for clinical therapeutic purposes. In this review, we will summarize up-to-date novel strategies for the committed differentiation into the three major glial cell types, i.e., astrocyte, oligodendrocyte, and microglia, from hiPSCs, focusing on the non-neuronal cell effects on the pathology of some representative neurological diseases. Furthermore, the application of hiPSC-derived glial cells in neurological disease modeling will be discussed, so as to gain further insights into the development of new therapeutic targets for treatment of neurological disorders.
- Published
- 2018
- Full Text
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10. Emerging Roles of Astrocytes in Neuro-Vascular Unit and the Tripartite Synapse With Emphasis on Reactive Gliosis in the Context of Alzheimer’s Disease
- Author
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Cai-Yun Liu, Yu Yang, Wei-Na Ju, Xu Wang, and Hong-Liang Zhang
- Subjects
astrocytes ,Alzheimer’s disease ,neuro-degeneration ,neuro-vascular unit ,synapse ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Astrocytes, which are five-fold more numerous than neurons in the central nervous system (CNS), are traditionally viewed to provide simple structural and nutritional supports for neurons and to participate in the composition of the blood brain barrier (BBB). In recent years, the active roles of astrocytes in regulating cerebral blood flow (CBF) and in maintaining the homeostasis of the tripartite synapse have attracted increasing attention. More importantly, astrocytes have been associated with the pathogenesis of Alzheimer’s disease (AD), a major cause of dementia in the elderly. Although microglia-induced inflammation is considered important in the development and progression of AD, inflammation attributable to astrogliosis may also play crucial roles. A1 reactive astrocytes induced by inflammatory stimuli might be harmful by up-regulating several classical complement cascade genes thereby leading to chronic inflammation, while A2 induced by ischemia might be protective by up-regulating several neurotrophic factors. Here we provide a concise review of the emerging roles of astrocytes in the homeostasis maintenance of the neuro-vascular unit (NVU) and the tripartite synapse with emphasis on reactive astrogliosis in the context of AD, so as to pave the way for further research in this area, and to search for potential therapeutic targets of AD.
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- 2018
- Full Text
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11. Sex-Related Overactivation of NLRP3 Inflammasome Increases Lethality of the Male COVID-19 Patients
- Author
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Hong-Liang Zhang, Yu-Jie Tang, and Jinhui Tao
- Subjects
Coronavirus disease 2019 (COVID-19) ,QH301-705.5 ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Inflammation ,Review ,Disease ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Biochemistry ,Asymptomatic ,medicine ,sex ,Molecular Biosciences ,Biology (General) ,Molecular Biology ,business.industry ,SARS-CoV-2 ,P2X7R ,COVID-19 ,Inflammasome ,medicine.disease ,NLRP3 inflammasome ,Immunology ,cytokine storm ,Lethality ,medicine.symptom ,Cytokine storm ,business ,medicine.drug - Abstract
The COVID-19 pandemic, caused by SARS-CoV-2 infection, remains a dramatic threat to human life and economic well-being worldwide. Significant heterogeneity in the severity of disease was observed for patients infected with SARS-CoV-2 ranging from asymptomatic to severe cases. Moreover, male patients had a higher probability of suffering from high mortality and severe symptoms linked to cytokine storm and excessive inflammation. The NLRP3 inflammasome is presumably critical to this process. Sex differences may directly affect the activation of NLRP3 inflammasome, impacting the severity of observed COVID-19 symptoms. To elucidate the potential mechanisms underlying sex based differences in NLRP3 activation during SARS-CoV-2 infection, this review summarizes the reported mechanisms and identifies potential therapeutic targets.
- Published
- 2021
12. Pharmacological Targeting of Microglial Activation: New Therapeutic Approach
- Author
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Caiyun Liu, Hong-Liang Zhang, Chang Liu, and Xu Wang
- Subjects
0301 basic medicine ,Chemokine ,Cell type ,microglia ,Inflammation ,Review ,neuroinflammation ,lcsh:RC321-571 ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,0302 clinical medicine ,Immune system ,medicine ,microglial activation ,lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry ,Reactive nitrogen species ,Neuroinflammation ,chemistry.chemical_classification ,Reactive oxygen species ,polarization ,biology ,Microglia ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,nervous system ,biology.protein ,Parkinson’s disease ,medicine.symptom ,Neuroscience ,030217 neurology & neurosurgery - Abstract
Mounting evidence suggests that neuroinflammation is not just a consequence but a vital contributor to the development and progression of Parkinson’s disease (PD). Microglia in particular, may contribute to the induction and modulation of inflammation in PD. Upon stimulation, microglia convert into activated phenotypes, which exist along a dynamic continuum and bear different immune properties depending on the disease stage and severity. Activated microglia release various factors involved in neuroinflammation, such as cytokines, chemokines, growth factors, reactive oxygen species (ROS), reactive nitrogen species (RNS), and prostaglandins (PGs). Further, activated microglia interact with other cell types (e.g., neurons, astrocytes and mast cells) and are closely associated with α-synuclein (α-syn) pathophysiology and iron homeostasis disturbance. Taken together, microglial activation and microglia-mediated inflammatory responses play essential roles in the pathogenesis of PD and elucidation of the complexity and imbalance of microglial activation may shed light on novel therapeutic approaches for PD.
- Published
- 2019
13. Blood Brain Barrier Permeability Could Be a Biomarker to Predict Severity of Neuromyelitis Optica Spectrum Disorders: A Retrospective Analysis
- Author
-
Jie Zhu, Hong-Liang Zhang, Ying Wang, Shuai Hou, Shuang Wang, Chao Lu, Jinming Han, Yang Chen, Mingqin Zhu, Wenjuan Lang, Dong Wang, Caiyun Liu, Nannan Zheng, Yu Zhang, and Yang Gao
- Subjects
0301 basic medicine ,Pathology ,medicine.medical_specialty ,blood brain barrier ,Blood–brain barrier ,lcsh:RC346-429 ,03 medical and health sciences ,0302 clinical medicine ,White blood cell ,medicine ,aquaporin 4 ,lcsh:Neurology. Diseases of the nervous system ,Original Research ,Expanded Disability Status Scale ,biology ,business.industry ,Albumin ,albumin index ,Spinal cord ,Myelin basic protein ,030104 developmental biology ,Aquaporin 4 ,medicine.anatomical_structure ,Neurology ,nervous system ,biology.protein ,cardiovascular system ,Biomarker (medicine) ,biomarker ,Neurology (clinical) ,neuromyelitis optica spectrum disorders ,business ,030217 neurology & neurosurgery - Abstract
Background: Blood-brain barrier (BBB) pathology exists in neuromyelitis optica spectrum disorders (NMOSD). However, the clinical use of BBB permeability, such as predicting disease severity of NMOSD, has rarely been studied in a large cohort of patients. Objectives: The current study explored the association between BBB permeability and clinical parameters in order to assess if BBB permeability could be a biomarker to predict disease severity and clinical characteristics of NMOSD. Methods: Among 69 enrolled NMOSD patients, 47 with albumin index over 5 × 10−3 were assigned to the increased BBB permeability group, and the remaining 22 were to the normal BBB permeability group. Disease severity was assessed using the Expanded Disability Status Scale (EDSS). Results: Patients in the increased BBB permeability group had significantly higher EDSS scores, anti-aquporin-4 immunoglobulin G titers, more dense cerebrospinal fluid protein concentrations, white blood cell counts, myelin basic protein levels and more dense complement 3 concentrations than found in the comparative normal BBB permeability group. The albumin index was positively correlated to the length of lesions in spinal cord. Conclusions: BBB permeability was associated with clinical features, laboratory results and radiological data of NMOSD patients, and may be a potential biomarker to predict disease severity and clinical characteristics of NMOSD.
- Published
- 2018
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