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The Micangshan-Dabashan tectonic belt, located in the southern Qinling-Dabie Orogen near the northeastern Tibetan Plateau, is a crucial area for understanding the processes and mechanisms of orogenesis. Previous studies have been focused on the cooling process via thermochronology and the mechanism and process of basement uplift have been investigated. However, the coupling process of basement exhumation and sedimentary cap cooling is unclear. The tectono-thermal history constrained by the detrital apatite fission track (AFT) results could provide valuable information for understanding crustal evolution and the coupling process. In this study, we provided new detrital AFT thermochronology results from the Micangshan-Dabashan tectonic belt and obtained nine high-quality tectono-thermal models revealing the Meso-Cenozoic cooling histories. The AFT ages and lengths suggest that the cooling events in the Micangshan area were gradual from north (N) to south (S) and different uplift occurred on both sides of Micangshan massif. The cooling in Dabashan tectonic zone was gradual from northeast (NS) to southwest (SW). The thermal histories show that a relatively rapid cooling since ca. 160 Ma occurred in the Micangshan-Dabashan tectonic belt, which was a response to the event of Qinling orogenic belt entered the intracontinental orogenic deformation. This cooling event may relate to the northeastward dextral compression of the Yangtze Block. The sedimentary cap of Cambriano-Ordovician strata responded positively to this rapid cooling event and entered the PAZ since ca. 63 Ma. The deep buried samples may be limited affected by climate and water erosion and the accelerated cooling was not obvious in the Late Cenozoic. Collectively, the cooling processes of basement and sedimentary cap in Micangshan-Dabashan tectonic belt were inconsistent. The uplift of the sedimentary area is not completely consistent with that of the basement under thrust and nappe action. The rigid basement was not always continuous and rapidly uplifted or mainly showed as lateral migration in a certain stage because of the different intensities and modes of thrust and nappe action, and the plastic sedimentary strata rapidly uplifted due to intense folding deformation.

2′-fucosyllactose (2′-FL), one of the simplest but most abundant oligosaccharides in human milk, has been demonstrated to have many positive benefits for the healthy development of newborns. However, the high-cost production and limited availability restrict its widespread use in infant nutrition and further research on its potential functions. In this study, on the basis of previous achievements, we developed a powerful cell factory by using a lacZ-mutant Escherichia coli C41 (DE3)ΔZ to ulteriorly increase 2′-FL production by feeding inexpensive glycerol. Initially, we co-expressed the genes for GDP-L-fucose biosynthesis and heterologous α-1,2-fucosyltransferase in C41(DE3)ΔZ through different plasmid-based expression combinations, functionally constructing a preferred route for 2′-FL biosynthesis. To further boost the carbon flux from GDP-L-fucose toward 2′-FL synthesis, deletion of chromosomal genes (wcaJ, nudD, and nudK) involved in the degradation of the precursors GDP-L-fucose and GDP-mannose were performed. Notably, the co-introduction of two heterologous positive regulators, RcsA and RcsB, was confirmed to be more conducive to GDP-L-fucose formation and thus 2′-FL production. Further a genomic integration of an individual copy of α-1,2-fucosyltransferase gene, as well as the preliminary optimization of fermentation conditions enabled the resulting engineered strain to achieve a high titer and yield. By collectively taking into account the intracellular lactose utilization, GDP-L-fucose availability, and fucosylation activity for 2′-FL production, ultimately a highest titer of 2′-FL in our optimized conditions reached 6.86 g/L with a yield of 0.92 mol/mol from lactose in the batch fermentation. Moreover, the feasibility of mass production was demonstrated in a 50-L fed-batch fermentation system in which a maximum titer of 66.80 g/L 2′-FL was achieved with a yield of 0.89 mol 2′-FL/mol lactose and a productivity of approximately 0.95 g/L/h 2′-FL. As a proof of concept, our preliminary 2′-FL production demonstrated a superior production performance, which will provide a promising candidate process for further industrial production.

Mortierella alpina (M. alpina) is well known for arachidonic acid (ARA) production. However, low efficiency and unstableness are long existed problems for industrial production of ARA by M. alpina due to the lack of online regulations. The aim of the present work is to develop an online-regulation strategy for efficient and stable ARA production in industry. The strategy was developed in 50 L fermenters and then applied in a 200 m3 fermenter. Results indicated that yeast extract (YE) highly increased cell growth in shake flask, it was then used in bioreactor fermentation by various feeding strategies. Feeding YE to control respiratory quotient (RQ) at 1.1 during 0–48 h and at 1.5 during 48–160 h, dry cell weight, and ARA titer reached 53.1 and 11.49 g/L in 50 L fermenter, which were increased by 79.4 and 36.9% as compared to that without YE feeding, respectively. Then, the online RQ-feedback strategy was applied in 200 m3 bioreactor fermentation and an average ARA titer of 16.82 g/L was obtained from 12 batches, which was 41.0% higher than the control batches. This is the first report on successful application of online RQ-feedback control of YE in ARA production, especially in an industrial scale of 200 m3 fermentation. It could be applied to other industrial production of microbial oil by oleaginous microorganisms.