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Despite Northeastern India being “Treasure House of Citrus Genetic Wealth,” genetic erosion of citrus diversity poses severe concern with a corresponding loss in seed microbial diversity. The seed microbiome of citrus species unique to the Purvanchal Himalaya is seldom explored for their use in sustainable orchard management. Isolation and characterization of culturable seed microbiomes of eight citrus species, namely, Citrus reticulata Blanco, C. grandis (L.) Osbeck, C. latipes Tanaka, C. megaloxycarpa Lushaigton, C. jambhiri Lush, C. sinensis (L.) Osbeck, C. macroptera Montr, and C. indica Tanaka collected from NE India were carried out. The isolates were then screened for an array of plant growth–promoting (PGP) traits [indole acetic acid (IAA) production, N2 fixation, phosphate and zinc complex dissolution, siderophores, and Hydrogen Cyanide (HCN) production]. The pure culture isolates of seed microbiomes were capable of dissolving insoluble Ca3(PO4)2 (1.31–4.84 µg Pi ml-1 h-1), Zn3(PO4)2 (2.44–3.16 µg Pi ml-1 h-1), AlPO4 (1.74–3.61 µg Pi ml-1 h-1), and FePO4 (1.54–4.61µg Pi ml-1 h-1), mineralized phytate (12.17–18.00 µg Pi ml-1 h-1) and produced IAA-like substances (4.8–187.29 µg ml-1 h-1). A few isolates of the seed microbiome were also able to fix nitrogen, secrete siderophore-like compounds and HCN, and dissolve ZnSO4 and ZnO. The 16S ribosomal Ribonucleic Acid (rRNA)–based taxonomic findings revealed that Bacillus was the most dominant genus among the isolates across citrus species. Isolates CG2-1, CME6-1, CME6-4, CME6-5, CME6-9, CJ7-1, CMA10-1, CI11-3, and CI11-4 were identified as promising bioinoculants for development of microbial consortium having multifaceted PGP traits for nutritional benefits of nitrogen, phosphorus and zinc, and IAA hormonal benefits to citrus crops for better fitness in acid soils.

IntroductionMillets are nutritionally superior and climate-resilient short-duration crops and hold a prominent place in cropping sequences around the world. They have immense potential to grow in a marginal environment due to diverse adaptive mechanisms.MethodsAn experiment was conducted in an organic production system in the North Eastern Himalayan foothills of India for 3 consecutive years by evaluating high-yielding varieties (HYVs) of different millets, viz., finger millet, foxtail millet, little millet, barnyard millet, proso millet, and browntop millet, along with local landraces of finger millets (Sikkim-1 and Sikkim-2; Nagaland-1 and Nagaland-2) to identify stable, high-yielding, and nutritionally superior genotypes suited for the region.ResultsAmong the various millets, finger millet, followed by little millet and foxtail millet, proved their superiority in terms of productivity (ranging between 1.16 and 1.43 Mg ha−1) compared to other millets. Among different varieties of finger millets, cv. VL Mandua 352 recorded the highest average grain yield (1.43 Mg ha−1) followed by local landraces, Nagaland-2 (1.31 Mg ha−1) and Sikkim-1 (1.25 Mg ha−1). Root traits such as total root length, root volume, average diameter of roots, and root surface area were significantly higher in finger millet landraces Nagaland-1, Nagaland-2, and Sikkim-1 compared to the rest of the millet genotypes. The different millets were found to be rich sources of protein as recorded in foxtail millet cv. SiA 3088 (12.3%), proso millet cv. TNAU 145 (11.5%), and finger millet landraces, Sikkim-1 and Nagaland-2 (8.7% each). Finger millet landrace Sikkim-2 recorded the highest omega-6 content (1.16%), followed by barnyard millet cv. VL 207 (1.09%). Barnyard millet cv. VL 207 recorded the highest polyunsaturated fatty acid (PUFA) content (1.23%), followed by foxtail millet cv. SiA 3088 (1.09%). The local finger millet landraces Sikkim-1 and Sikkim-2 recorded the highest levels of histidine (0.41%) and tryptophan (0.12%), respectively. Sikkim-1 and Nagaland-2 recorded the highest level of thiamine (0.32%) compared to the HYVs.ConclusionThese findings indicate that finger millet has great potential in the organic production system of the North Eastern Himalayan Region (NEHR) of India, and apart from HYVs like VL Mandua 352, local landraces, viz., Nagaland-2 and Sikkim-1, should also be promoted for ensuring food and nutritional security in this fragile ecosystem.

IntroductionOrganic farming is a promising solution for mitigating environmental burdens related to input-intensive agricultural practices. The major challenge in organic agriculture is the non-availability of large quantities of organic inputs required for crop nutrition and sustaining soil health, which can be resolved by efficient recycling of the available on- and off-farm resources and the integration of the components as per the specific locations.MethodsAn integrated organic farming system (IOFS) model comprising agricultural and horticultural crops, rainwater harvesting units, livestock components, and provisions for nutrient recycling was developed and disseminated in the adopted organic villages Mynsain, Pynthor, and Umden Umbathiang in the Ri-Bhoi District, Meghalaya, India, to improve the income and livelihood of farmers. Harvested rainwater in farm ponds and Jalkunds was used for live-saving irrigation in the winter months and diversified homestead farming activities, such as growing high-value crops and rearing cattle, pigs, and poultry.ResultsMaize, french bean, potato, ginger, tomato, carrot, and chili yields in the IOFS model increased by 20%−30%, 40%−45%, 25%−30%, 33%−40%, 45%−50%, 37%−50%, and 27%−30%, respectively, compared with traditional practices. Some farmers produced vermicompost in vermibeds (made of high-density polyethylene) and cement brick chambers, generating 0.4−1.25 tons per annum. Two individual farmers, Mr. Jrill Makroh and Mrs. Skola Kurbah obtained net returns (without premium price) of Rs. 46,695 ± 418 and Rs. 31,102 ± 501 from their respective 0.27- and 0.21-ha IOFS models, which is equivalent to Rs. 172,944 ± 1,548/ha/year and Rs. 148,105 ± 2,385/ha/year, respectively. The net returns obtained from the IOFS models were significantly higher than those obtained from the farmers' practice of maize-fallow or cultivation of maize followed by vegetable (~30% of the areas). It is expected that, with the certification of organic products, the income and livelihood of the farmers will improve further over the years. While Mr. Jrill Makroh's model supplied 95.1%, 82.0%, and 96.0% of the total N, P2O5, and K2O, respectively, needed by the system, Mrs. Skola Kurbah's model supplied 76.0%, 68.6%, and 85.5% of the total N, P2O5, and K2O, respectively.DiscussionThus, IOFS models should be promoted among hill farmers so that they can efficiently recycle farm resources and increase their productivity, net returns, and livelihood while reducing their dependence on external farm inputs.

The eastern Himalayas, one of the important hotspots of global biodiversity, have a rich diversity of wild edible fruit trees. The fruits of these tree species have been consumed by the tribal people since time immemorial. However, there is limited information available on the biochemical and antioxidant properties of the fruits. Therefore, the present investigation was undertaken to study the physico-chemical and antioxidant properties of the nine most important wild fruit trees. Among the species, Pyrus pashia had the maximum fruit weight (37.83 g), while the highest juice (43.72%) and pulp content (84.67%) were noted in Haematocarpus validus and Myrica esculenta, respectively. Maximum total soluble solids (18.27%), total sugar (11.27%), moisture content (88.39%), ascorbic acid content (63.82 mg/100 g), total carotenoids (18.47 mg/100 g), and total monomeric anthocyanin (354.04 mg/100 g) were recorded in H. validus. Docynia indica had the highest total phenolic content (19.37 mg GAE/g), while H. validus recorded the highest total flavonoids and flavanol content. The antioxidant activities of the different fruits ranged from 0.17 to 0.67 IC50 for DPPH activity and 3.59–13.82 mg AAE/g for FRAP. These fruits had attractive pigmentation of both pulp and juice and were a good potential source for the extraction of natural edible color in the food industry. The fruits also possess high market prices; Prunus nepalensis fetched $ 34.10–$ 141.5 per tree. Therefore, these fruits are rich sources of antioxidants, pigments and have a high market value for livelihood and nutritional security.