1. The proteomic fingerprint in infants with single ventricle heart disease in the interstage period: evidence of chronic inflammation and widespread activation of biological networks
- Author
-
Lindsay M. Thomson, Christopher A. Mancuso, Kelly R. Wolfe, Ludmila Khailova, Sierra Niemiec, Eiman Ali, Michael DiMaria, Max Mitchell, Mark Twite, Gareth Morgan, Benjamin S. Frank, and Jesse A. Davidson
- Subjects
biomarkers ,congenital heart disease ,congenital heart defect ,Glenn ,hypoplastic left heart syndrome ,inflammation ,Pediatrics ,RJ1-570 - Abstract
IntroductionChildren with single ventricle heart disease (SVHD) experience significant morbidity across systems and time, with 70% of patients experiencing acute kidney injury, 33% neurodevelopmental impairment, 14% growth failure, and 5.5% of patients suffering necrotizing enterocolitis. Proteomics is a method to identify new biomarkers and mechanisms of injury in complex physiologic states.MethodsInfants with SVHD in the interstage period were compared to similar-age healthy controls. Serum samples were collected, stored at −80°C, and run on a panel of 1,500 proteins in single batch analysis (Somalogic Inc., CO). Partial Least Squares-Discriminant Analysis (PLS-DA) was used to compare the proteomic profile of cases and controls and t-tests to detect differences in individual proteins (FDR 500 circulating proteins distinguishing the groups. These proteomic data identify widespread protein dysregulation across multiple biologic systems with promising biological plausibility as drivers of SVHD morbidity.
- Published
- 2023
- Full Text
- View/download PDF