1. A Guinea Pig Model of Airway Smooth Muscle Hyperreactivity Induced by Chronic Allergic Lung Inflammation: Contribution of Epithelium and Oxidative Stress
- Author
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Luiz Henrique César Vasconcelos, Maria da Conceição Correia Silva, Alana Cristina Costa, Giuliana Amanda de Oliveira, Iara Leão Luna de Souza, Fernando Ramos Queiroga, Layanne Cabral da Cunha Araujo, Glêbia Alexa Cardoso, Renato Fraga Righetti, Alexandre Sérgio Silva, Patrícia Mirella da Silva, Carla Roberta de Oliveira Carvalho, Giciane Carvalho Vieira, Iolanda de Fátima Lopes Calvo Tibério, Fabiana de Andrade Cavalcante, and Bagnólia Araújo da Silva
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Inflammation ,medicine.disease_cause ,Guinea pig ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,relaxation ,Internal medicine ,medicine ,oxidative stress ,Pharmacology (medical) ,Original Research ,Pharmacology ,Lung ,biology ,business.industry ,lcsh:RM1-950 ,contractile reactivity ,asthma ,respiratory system ,medicine.disease ,Epithelium ,Ovalbumin ,lcsh:Therapeutics. Pharmacology ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,chemistry ,Bronchial hyperresponsiveness ,030220 oncology & carcinogenesis ,biology.protein ,medicine.symptom ,airways ,business ,Oxidative stress ,Histamine - Abstract
Asthma is a heterogeneous disease of the airways characterized by chronic inflammation associated with bronchial and smooth muscle hyperresponsiveness. Currently, different murine models for the study of asthma show poor bronchial hyperresponsiveness due to a scarcity of smooth muscle and large airways, resulting in a failure to reproduce smooth muscle hyperreactivity. Thus, we aimed to standardize a guinea pig model of chronic allergic lung inflammation mimicking airway smooth muscle hyperreactivity observed in asthmatics (Asth). Animals were randomly divided into a control group (Ctrl), which received saline (0.9% NaCl), and the Asth group, subjected to in vivo sensitization with ovalbumin (OVA) nebulization. Morphological analysis was performed by hematoxylin-eosin staining. Bronchial hyperresponsiveness was evaluated by nebulization time in the fifth, sixth, and seventh inhalations (NT5-7) and tracheal isometric contractions were assessed by force transducer. Total antioxidant capacity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and protein expression by Western blot. Histologically, the Asth group developed peribronchial cellular infiltrate, epithelial hyperplasia and smooth muscle thickening. After the fourth nebulization, the Asth group developed bronchial hyperreactivity. The trachea from the Asth group contracted after in vitro stimulation with OVA, differing from the Ctrl group, which showed no response. Additionally, airway smooth muscle hyperreactivity to carbachol and histamine was observed in the Asth group only in intact epithelium preparations, but not to KCl, and this effect was associated with an augmented production of reactive oxygen species. Moreover, lung inflammation impaired the relaxant potency of isoproterenol only in intact epithelium preparations, without interfering with nifedipine, and it was found to be produced by transforming growth factor-β negative modulation of β adrenergic receptors and, furthermore, big-conductance Ca2+-sensitive K+ channels. These effects were also associated with increased levels of phosphatidylinositol 3-kinases but not extracellular signal-regulated kinases 1/2 or phosphorylation, and augmented α-actin content as well, explaining the increased smooth muscle mass. Furthermore, pulmonary antioxidant capacity was impaired in the Asth group. Therefore, we developed a standardized and easy-to-use, reproducible guinea pig model of lung inflammation that mimics airway smooth muscle hypercontractility, facilitating the investigation of the mechanisms of bronchial hyperresponsiveness in asthma and new therapeutic alternatives.
- Published
- 2019
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