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Fillet texture plays a significant role in consumer acceptance, which heavily influenced by exercise according to previous studies. “Xianfeng No. 2”, a novel hybrid of Megalobrama amblycephala (M. amblycephala) (♀) and Ancherythroculter nigrocauda (A. nigrocauda) (♂), with characteristics of quick growth, good disease resistance, and anti-stress capability, is one of an important aquaculture fish species in China, however, the ways to improve fillet quality in this species remains unknown. In this study, a short-term exercise trial (0-16d), hematoxylin and eosin (H&E) staining, and texture analysis were carried out to determine the effects of exercise on muscle cellularity and texture. Furthermore, 16S rRNA analysis was performed to determine the impacts of exercise on the intestinal microbiota. Compared with non-exercised group (0d), exercised groups showed influences in muscle cellularity (decreased muscle fiber diameter and increased muscle fiber density) and improvements in muscle texture (increased hardness, cohesiveness, gumminess, and chewiness). In addition, 16S rRNA sequencing analysis exhibited that short-term exercise could significantly increase the community richness and diversity, as well as alter the composition of gut microflora in this hybrid species. At the level of phylum, the relative richness of Proteobacteria, Firmicutes, Bacteroidetes, and Fusobacteria changed remarkably after short-term exercise. At the level of genus, the relative richness of Pseudomonas, Acinetobacter, Stenotrophomonas, Alpinimonas, Rhodococcus, Shewanella, Brevundimonas changed significantly, suggested that short-term swimming exercise might increase intrinsic antimicrobial resistance and promote denitrification capacity of this hybrid species. Furthermore, a LEfSe analysis of intestinal microflora communities illustrated that Pseudomonas and MNG7 may be utilized to distinguish between exercised and non-exercised fish. Our research, collectively, found an effective way to improve fillet texture by exercise, revealed the impacts of exercise on the intestinal microbiota, further expanded our knowledge of fish under exercise.

Gastric cancer (GC) has a high incidence worldwide, and when detected, the majority of patients have already progressed to advanced stages. Long non-coding RNAs (lncRNAs) have a wide range of biological functions and affect tumor occurrence and development. However, the potential role of lncRNAs in GC diagnosis remains unclear. We selected five high-quality samples from each group of chronic non-atrophic gastritis, gastric mucosal intraepithelial neoplasia, and GC tissues for analysis. RNA-seq was used to screen the differentially expressed lncRNAs, and we identified 666 differentially expressed lncRNAs between the chronic non-atrophic gastritis and GC groups, 13 differentially expressed lncRNAs between the gastric mucosal intraepithelial neoplasia and GC groups, and 507 differentially expressed lncRNAs between the chronic non-atrophic gastritis and gastric mucosal intraepithelial neoplasia groups. We also identified six lncRNAs (lncRNA H19, LINC00895, lnc-SRGAP2C-16, lnc-HLA-C-2, lnc-APOC1-1, and lnc-B3GALT2-1) which not only differentially expressed between the chronic non-atrophic gastritis and GC groups, but also differentially expressed between the gastric mucosal intraepithelial neoplasia and GC groups. Furthermore, RT-qPCR was used to verify the differentially co-expressed lncRNAs. LncSEA was used to conduct a functional analysis of differentially expressed lncRNAs. We also predicted the target mRNAs of the differentially expressed lncRNAs through bioinformatics analysis and analyzed targeting correlations between three differentially co-expressed lncRNAs and mRNAs (lncRNA H19, LINC00895, and lnc-SRGAP2C-16). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases were used to explore the functions of target mRNAs of differentially expressed lncRNAs. In conclusion, our study provides a novel perspective on the potential functions of differentially expressed lncRNAs in GC occurrence and development, indicating that the differentially expressed lncRNAs might be new biomarkers for early GC diagnosis.