1. New Reassortant H5N6 Highly Pathogenic Avian Influenza Viruses in Southern China, 2014
- Author
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Hui Song, Jin Cui, Ming Liao, Nannan Qu, Siyu Wu, Peirong Jiao, Guowen Ouyang, Zhishan Zhao, Nianchen Wang, and Yafen Song
- Subjects
0301 basic medicine ,Microbiology (medical) ,medicine.medical_specialty ,animal structures ,animal diseases ,viruses ,030106 microbiology ,lcsh:QR1-502 ,Biology ,medicine.disease_cause ,Microbiology ,H5N1 genetic structure ,lcsh:Microbiology ,Virus ,Duck ,03 medical and health sciences ,Phylogenetics ,Molecular genetics ,evolution ,ducks ,medicine ,pathogenicity ,Clade ,Gene ,Original Research ,Phylogenetic tree ,highly pathogenic avian influenza virus ,virus diseases ,H5N6 ,Virology ,Influenza A virus subtype H5N1 ,030104 developmental biology - Abstract
New reassortant H5N6 highly pathogenic avian influenza viruses were isolated from apparently healthy domestic ducks in Southern China in 2014. Our results show that the viruses grew efficiently in eggs and replicated systemically in chickens. They were completely lethal in chicken (100% mortality), and the mean death time (MDT) was 6 to 7 days post-inoculation (DPI). The viruses could transmit in chickens by naïve contact. BLAST analysis revealed that their HA gene was most closely related to A/wild duck/Shangdong/628/2011 (H5N1), and their NA genes were most closely related to A/swine/Guangdong/K6/2010 (H6N6). The other genes had the highest identity with A/wild duck/Fujian/1/2011(H5N1). The results of phylogenetic analysis showed that their HA genes clustered into clade 2.3.4.4 of the H5N1 viruses and all genes derived from H5 were Mix-like or H6-like viruses. Thus, the new H5N6 viruses were reassortanted of H5N1 and H6N6 virus. Therefore, the circulation of the new H5N6 avian influenza viruses may become a threat to poultry and human health.
- Published
- 2016