1. SERS-Based Evaluation of the DNA Methylation Pattern Associated With Progression in Clonal Leukemogenesis of Down Syndrome
- Author
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Vlad Moisoiu, Valentina Sas, Andrei Stefancu, Stefania D. Iancu, Ancuta Jurj, Sergiu Pasca, Sabina Iluta, Alina-Andreea Zimta, Adrian B. Tigu, Patric Teodorescu, Cristina Turcas, Cristina Blag, Delia Dima, Gheorghe Popa, Smaranda Arghirescu, Sorin Man, Anca Colita, Nicolae Leopold, and Ciprian Tomuleasa
- Subjects
down syndrome ,Histology ,myeloproliferative neoplasm ,Biomedical Engineering ,Bioengineering ,Context (language use) ,02 engineering and technology ,transient leukemia associated with down syndrome ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,acute leukemia ,Myeloproliferative neoplasm ,Acute leukemia ,SERS ,Chemistry ,Area under the curve ,Bioengineering and Biotechnology ,Methylation ,021001 nanoscience & nanotechnology ,medicine.disease ,Molecular biology ,Leukemia ,030220 oncology & carcinogenesis ,Perspective ,DNA methylation ,0210 nano-technology ,TP248.13-248.65 ,DNA ,Biotechnology - Abstract
Here we show that surface-enhanced Raman scattering (SERS) analysis captures the relative hypomethylation of DNA from patients with acute leukemia associated with Down syndrome (AL-DS) compared with patients diagnosed with transient leukemia associated with Down syndrome (TL-DS), an information inferred from the area under the SERS band at 1005 cm–1 attributed to 5-methycytosine. The receiver operating characteristic (ROC) analysis of the area under the SERS band at 1005 cm–1 yielded an area under the curve (AUC) of 0.77 in differentiating between the AL-DS and TL-DS groups. In addition, we showed that DNA from patients with non-DS myeloproliferative neoplasm (non-DS-MPN) is hypomethylated compared to non-DS-AL, the area under the SERS band at 1005 cm–1 yielding an AUC of 0.78 in separating between non-DS-MPN and non-DS-AL. Overall, in this study, the area of the 1005 cm–1 DNA SERS marker band shows a stepwise decrease in DNA global methylation as cells progress from a pre-leukemia to a full-blown acute leukemia, highlighting thus the potential of SERS as an emerging method of analyzing the methylation landscape of DNA in the context of leukemia genesis and progression.
- Published
- 2021