1. Blood DNA methylation marks discriminate Chagas cardiomyopathy disease clinical forms.
- Author
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Brochet P, Ianni B, Nunes JPS, Frade AF, Teixeira PC, Mady C, Ferreira LRP, Kuramoto A, Pissetti CW, Saba B, Cândido DDS, Dias F, Sampaio M, Marin-Neto JA, Fragata A, Zaniratto RCF, Siqueira S, Peixoto GDL, Rigaud VOC, Buck P, Almeida RR, Lin-Wang HT, Schmidt A, Martinelli M, Hirata MH, Donadi E, Rodrigues Junior V, Pereira AC, Kalil J, Spinelli L, Cunha-Neto E, and Chevillard C
- Subjects
- Adenosine Triphosphate metabolism, Biomarkers metabolism, DNA Methylation, Humans, Chagas Cardiomyopathy diagnosis, Chagas Cardiomyopathy genetics, Chagas Disease genetics
- Abstract
Chagas disease is a parasitic disease from South America, affecting around 7 million people worldwide. Decades after the infection, 30% of people develop chronic forms, including Chronic Chagas Cardiomyopathy (CCC), for which no treatment exists. Two stages characterized this form: the moderate form, characterized by a heart ejection fraction (EF) ≥ 0.4, and the severe form, associated to an EF < 0.4. We propose two sets of DNA methylation biomarkers which can predict in blood CCC occurrence, and CCC stage. This analysis, based on machine learning algorithms, makes predictions with more than 95% accuracy in a test cohort. Beyond their predictive capacity, these CpGs are located near genes involved in the immune response, the nervous system, ion transport or ATP synthesis, pathways known to be deregulated in CCCs. Among these genes, some are also differentially expressed in heart tissues. Interestingly, the CpGs of interest are tagged to genes mainly involved in nervous and ionic processes. Given the close link between methylation and gene expression, these lists of CpGs promise to be not only good biomarkers, but also good indicators of key elements in the development of this pathology., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Brochet, Ianni, Nunes, Frade, Teixeira, Mady, Ferreira, Kuramoto, Pissetti, Saba, Cândido, Dias, Sampaio, Marin-Neto, Fragata, Zaniratto, Siqueira, Peixoto, Rigaud, Buck, Almeida, Lin-Wang, Schmidt, Martinelli, Hirata, Donadi, Rodrigues Junior, Pereira, Kalil, Spinelli, Cunha-Neto and Chevillard.)
- Published
- 2022
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