1. Pro-inflammatory Analysis of Macrophages in Contact with Titanium Particles and Porphyromonas gingivalis
- Author
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Jacqueline Abranches, Cindy Goes Dodo, Alejandro Avilés-Reyes, Luiz Augusto Duarte Meirelles, Karina Gonzalez Silvério Ruiz, and Altair Antoninha Del Bel Cury
- Subjects
0301 basic medicine ,Lipopolysaccharide ,medicine.medical_treatment ,Cell ,Gene Expression ,Enzyme-Linked Immunosorbent Assay ,Real-Time Polymerase Chain Reaction ,Cell morphology ,Article ,Cell Line ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Humans ,titanium ,Viability assay ,Particle Size ,General Dentistry ,Porphyromonas gingivalis ,Dental Implants ,Titanium ,dental implant ,biology ,Macrophages ,O Antigens ,030206 dentistry ,biology.organism_classification ,Molecular biology ,cytokines ,030104 developmental biology ,medicine.anatomical_structure ,Cytokine ,chemistry ,Cell culture ,Microscopy, Electron, Scanning ,Cytokines ,Tumor necrosis factor alpha ,Inflammation Mediators ,osteolysis ,bone resorption - Abstract
During insertion of titanium dental implants, particles may shear from the implant to the periimplant region causing osteolysis, and their association with bacteria can exacerbate the inflammatory reaction. However, the association of a high invasive bacterium from the oral cavity, Porphyromonas gingivalis (Pg), and titanium particles remains unknown. This study evaluated pro-inflammatory reaction of human macrophages in contact with micro and nanoparticles of titanium associated with Porphyromonas gingivalis lipopolysaccharide (PgLPS). THP-1 cell were used and treated for 12, 24 and 48 h following 6 groups: Control(C), PgLPS (L); Microparticles (M); Nanoparticles (N); PgLPS and microparticles (LM); PgLPS and nanoparticles (LN). The following assays were carried out: i) cell viability using MTS, ii) cell morphology by SEM and iii) expression of tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) by qRT-PCR and ELISA. For statistics two-way ANOVA followed by Tukey’s test was used (p
- Published
- 2017