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With the increase in demand for wood and wood products, there is need to find alternative sources for the production of pulp and paper. Non wood plants have attracted interest due to their short growth cycles, moderate irrigation and fertilizer requirements and low lignin content. This study aimed at investigating the fibre characteristics of Agave sisalana for pulp and paper production. Leaves were collected from three sisal plant stands in Oyo State, Nigeria. The plant whorl was divided systematically into bottom, middle and top from which five leaves were randomly collected from each whorl position. The fibres of the leaves were extracted from which 2cm of fibres were taken from three positions on the leaf (top, middle and base). Extracted fibres were macerated and 3375 fibres were measured. The result shows that the fibre length of sisal increased with increasing length of the leaves from base to top with a mean value of 1.68mm. The fibre diameter and cell wall thickness averaged 16.98µm and 2.32µm respectively decreasing from the stem base to the top. The slenderness ratio averaged 99.4 while the coefficient of flexibility averaged 72.61. The highest value of the runkel ratio of Agave sisalana was 0.42. The fibres from sisal (Agave sisalana) leaves possess good fibre dimensions and derived values which will make it suitable for the production of high quality paper. [ABSTRACT FROM AUTHOR]