1. Pure platelet-rich plasma and supernatant of calcium-activated P-PRP induce different phenotypes of human macrophages
- Author
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Gisselle Escobar, Alejandro Escobar, María Carolina Ortiz, Claudio A. Perez, Mercedes N. López, Fabian Tempio, and Gabriel Ascui
- Subjects
Adult ,Male ,0301 basic medicine ,Embryology ,Adolescent ,animal diseases ,medicine.medical_treatment ,Biomedical Engineering ,chemistry.chemical_element ,Stimulation ,Calcium ,03 medical and health sciences ,0302 clinical medicine ,Antigens, CD ,medicine ,Humans ,Platelet ,Secretion ,Cells, Cultured ,CD86 ,Platelet-Rich Plasma ,Macrophages ,Middle Aged ,Molecular biology ,Interleukin-10 ,nervous system diseases ,Interleukin 10 ,030104 developmental biology ,Cytokine ,chemistry ,030220 oncology & carcinogenesis ,Platelet-rich plasma ,Female - Abstract
Aim: This study aimed to evaluate the effect of two platelet preparations used in the clinic, pure platelet-rich plasma (P-PRP) and the supernatant of calcium-activated P-PRP (S-PRP), on the phenotype of human macrophages. Materials & methods: Surface markers and cytokine production of human monocyte-derived macrophages were analyzed after 24 h stimulation with P-PRP or S-PRP. Results: P-PRP and S-PRP present no difference in the expression of CD206, a M2 tissue-repair macrophage-related marker. However, these same macrophages presented different levels of CD163, CD86 as well as different IL-10 secretion capacities after 24 h incubation. Conclusion: These platelet preparations do not have an equivalent biological effect over macrophages, which suggest that they may present different clinical regenerative potentials.
- Published
- 2018
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