1. Naphtho[1,2-b]furan-4,5-dione is a potent anti-MRSA agent against planktonic, biofilm and intracellular bacteria
- Author
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Jia-You Fang, Feng-Lin Yen, Shih-Chun Yang, Yi-Han Weng, Pei-Wen Wang, Ibrahim A. Aljuffali, and Chih-Hua Tseng
- Subjects
Keratinocytes ,Methicillin-Resistant Staphylococcus aureus ,Proteomics ,0301 basic medicine ,Microbiology (medical) ,Staphylococcus aureus ,food.ingredient ,Cell Survival ,Neutrophils ,Citric Acid Cycle ,Microbial Sensitivity Tests ,medicine.disease_cause ,Microbiology ,03 medical and health sciences ,Minimum inhibitory concentration ,food ,Bacterial Proteins ,Microscopy, Electron, Transmission ,medicine ,Humans ,Agar ,Agar diffusion test ,Furans ,Oxacillin ,Microbial Viability ,Minimum bactericidal concentration ,Chemistry ,Macrophages ,Cell Membrane ,Gluconeogenesis ,Biofilm ,Vancomycin Resistance ,Staphylococcal Infections ,Antimicrobial ,Anti-Bacterial Agents ,Molecular Docking Simulation ,030104 developmental biology ,Biofilms ,Pseudomonas aeruginosa ,Intracellular ,Naphthoquinones - Abstract
Aim: Naphtho[1,2-b]furan-4,5-dione (N12D) and naphtho[2,3-b]furan-4,9-dione (N23D) are furanonaphthoquinone derivatives from natural resources. We examined the antimicrobial activity of N12D and N23D against drug-resistant Staphylococcus aureus. Materials & methods: Minimum inhibitory concentration, minimum bactericidal concentration, bacterial viability and agar diffusion assay were conducted against methicillin-resistant S. aureus (MRSA) and clinical isolates of vancomycin-resistant S. aureus. Results & conclusion: The minimum inhibitory concentration of N12D and N23D against MRSA was 4.9–9.8 and 39 μM, respectively. With regard to the agar diffusion test, the inhibition zone of the quinone compounds was threefold larger than that of oxacillin. N12D was found to inhibit MRSA biofilm thickness from 24 to 16 μm as observed by confocal microscopy. N12D showed a significant reduction of the intracellular MRSA burden without decreasing the macrophage viability. The antibacterial mechanisms of N12D may be bacterial wall/membrane damage and disturbance of gluconeogenesis and the tricarboxylic acid cycle.
- Published
- 2017
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