1. Poly(ε-caprolactone)–carbon nanotube composite scaffolds for enhanced cardiac differentiation of human mesenchymal stem cells
- Author
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Spencer W. Crowder, Chee Chew Lim, Yi Liang, Simon Maltais, Peter N. Pintauro, Andrew M. Park, Hak-Joon Sung, Rutwik Rath, William H. Hofmeister, and Xintong Wang
- Subjects
Materials science ,Polyesters ,Cellular differentiation ,Cardiac differentiation ,Composite number ,Biomedical Engineering ,Medicine (miscellaneous) ,Bioengineering ,Nanotechnology ,Carbon nanotube ,Development ,Article ,law.invention ,chemistry.chemical_compound ,Tissue engineering ,law ,Humans ,General Materials Science ,Cells, Cultured ,Tissue Engineering ,Tissue Scaffolds ,Nanotubes, Carbon ,Mesenchymal stem cell ,Electrically conductive ,Cell Differentiation ,Mesenchymal Stem Cells ,chemistry ,Caprolactone ,Biomedical engineering - Abstract
Aim: To evaluate the efficacy of electrically conductive, biocompatible composite scaffolds in modulating the cardiomyogenic differentiation of human mesenchymal stem cells (hMSCs). Materials & methods: Electrospun scaffolds of poly(ε-caprolactone) with or without carbon nanotubes were developed to promote the in vitro cardiac differentiation of hMSCs. Results: Results indicate that hMSC differentiation can be enhanced by either culturing in electrically conductive, carbon nanotube-containing composite scaffolds without electrical stimulation in the presence of 5-azacytidine, or extrinsic electrical stimulation in nonconductive poly(ε-caprolactone) scaffolds without carbon nanotube and azacytidine. Conclusion: This study suggests a first step towards improving hMSC cardiomyogenic differentiation for local delivery into the infarcted myocardium. Original submitted 23 July 2012; Revised submitted 31 October 2012; Published online 27 March 2013
- Published
- 2013
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