Search

Your search keyword '"Fernström, E."' showing total 1 results
Start Over Author "Fernström, E." Publisher future science

Refine your results

1 results on '"Fernström, E."'

1. In-solution staining and arraying method for the immunofluorescence detection of γH2AX foci optimized for clinical applications.

Author

Johansson P, Muslimovic A, Hultborn R, Fernström E, and Hammarsten O

Subjects
Aminoglycosides pharmacology, Enediynes pharmacology, Fibroblasts metabolism, Humans, Leukocytes, Mononuclear drug effects, Leukocytes, Mononuclear radiation effects, Staining and Labeling methods, DNA Breaks, Double-Stranded, Fluorescent Antibody Technique methods, Histones analysis, Leukocytes, Mononuclear chemistry, Neoplasms radiotherapy, Tissue Array Analysis methods
Abstract

Immunofluorescence quantification of γH2AX foci is a powerful approach to quantify DNA double-strand breaks induced by cancer therapy or accidental exposure to ionizing radiation. Here we report a modification to the γH2AX immunofluorescence labeling method, whereby cells are stained in-solution before being spotted and fixed onto microscope slides. Our modified method allows arraying of 16 patient samples/slide ready for foci counting in 2 h and demonstrated reliably detection of γH2AX foci in mononuclear cells prepared from patients who had undergone radiation therapy.

Published
2011
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results