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Copyright of Turkish Journal of Hematology is the property of Galenos Yayinevi Tic. LTD. STI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

T cell subgroups are known to play important roles in the pathogenesis of allergic and non-allergic asthma. In this study the expression of the surface markers including CD4, CD8, CCR3, CCR4, CXCR3 and intracytoplasmic cytokine (IFN-g, IL-4 and IL-10) profiles of peripheral blood mononuclear cells in stimulated (PMA/ionomicin) and unstimulated conditions were investigated by flow cytometry. Stimulated Th cells increased their CXCR3+ IFN-g, CXCR3+ IL-4, CCR3+ IFN-g, CCR3+ IL-4, CCR3+ IL-10, CCR4+ IL-10 expressions in allergic asthma; but CXCR3+ IFN-g, CXCR3+ IL-4, CXCR3+ IL-10, CCR3+ IFN-g, CCR3+ IL-4 and CCR3+ IL-10 expression in non-allergic asthma. Cytotoxic T cells in allergic asthma expressed higher CCR3+ IFN-g, CCR3+ IL-4, CCR3+ IL-10, CCR4+ IL-4, CCR4+ IL-10 after stimulation. On the other hand CD8+ CXCR3+ IFN-g and CD8+ CXCR3+ IL-10 expressions increased in non-allergic asthma under the same stimulation. In our study T cells tended to elevate their intracellular cytokine levels in all study groups under both conditions. Comparing patients with allergic and non-allergic asthma, CCR3+ and CCR4+ Type 2 helper T cells produced higher amounts of IL-10 compared to Type 1 CD4+ CXCR3+ cells in allergic asthma. CD4+ CCR4+ cells expressed higher IFN-g and IL-10 levels without any stimulation in patients with allergic and non-allergic asthma respectively. In summary, our results indicate the role of T cells in the pathogenesis of allergic and non-allergic asthma, but also the complexity of the disease which can not be explained by using only the simple Th1/Th2 paradigma. [ABSTRACT FROM AUTHOR]