1. Agrobacterium-mediated transformation of tomato with the ICE1 transcription factor gene
- Author
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X M Jiang, G Yang, Yuze Zhang, Z L Gao, J X Juan, Y D Duan, X H Yu, and Wei Li
- Subjects
Time Factors ,Agrobacterium ,Genetic Vectors ,Genes, Plant ,Polymerase Chain Reaction ,Microbiology ,Transformation, Genetic ,Solanum lycopersicum ,Kanamycin ,Botany ,Genetics ,Regeneration ,Arabidopsis thaliana ,Genetically modified tomato ,Molecular Biology ,Gene ,Electrophoresis, Agar Gel ,Expression vector ,biology ,fungi ,food and beverages ,General Medicine ,Plants, Genetically Modified ,biology.organism_classification ,Adaptation, Physiological ,Anti-Bacterial Agents ,Cold Temperature ,Transformation (genetics) ,Carbenicillin ,Catalase ,biology.protein ,Transcription Factor Gene ,Cotyledon ,Transcription Factors - Abstract
ICE1 genes play a very important role in plants in cold conditions. To improve the cold resistance of tomato, the ICE1 gene of Arabidopsis thaliana was used to construct the plant expression vector p3301-ICE1, and was overexpressed in tomato through Agrobacterium-mediated transformation. Five strains of resistant plants were obtained. PCR and half-quantitative results showed that the ICE1 gene was transferred to tomato; three strains tested positive. After low-temperature stress treatment, praline content and peroxide and catalase activities in the transgenic tomato plants were higher compared with non-transgenic controls, while malondialdehyde content was clearly lower.
- Published
- 2015
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