1. Construction of a BAC library for buckwheat genome research -An application to positional cloning of agriculturally valuable traits
- Author
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Clayton G. Campbell, Masashi Mori, Daiki Matsumoto, Ohmi Ohnishi, Yasuo Yasui, and Tatsuya Ota
- Subjects
Genetics ,Positional cloning ,Agamous ,food and beverages ,Locus (genetics) ,General Medicine ,Biology ,Genome ,law.invention ,Sequence-tagged site ,law ,Genomic library ,Molecular Biology ,Gene ,Polymerase chain reaction - Abstract
We have constructed a BAC library for common buckwheat Fagopyrum esculentum Moench. The library includes 142,005 clones with an average insert size of ~76 kb, equivalent to approximately a 7~8-fold coverage of the genome. Polymerase chain reaction based screening of the library with AGAMOUS and FLORICAULA/LEAFY primers, has identified 7 and 9 BACs, respectively, which are consistent with the genome coverage. This library represents the first large insert genomic library for F. esculentum and it can be served as a genetic resource facilitating agricultural, pharmacological, physiological, and evolutionary studies of the species. To demonstrate the utilization of the library for characterizing agriculturally valuable traits, we developed a sequence tagged site marker tightly linked to the dwarf E locus as well as to the self-incompatibility complex locus and screened the library to initiate positional cloning of the causative genes.
- Published
- 2008