1. Sulphated oligosaccharides as inhibitors of hyaluronidases from bovine testis, bee venom and Streptococcus agalactiae.
- Author
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Salmen S, Hoechstetter J, Käsbauer C, Paper DH, Bernhardt G, and Buschauer A
- Subjects
- Animals, Bee Venoms enzymology, Bees, Cattle, Enzyme Inhibitors administration & dosage, Enzyme Inhibitors therapeutic use, Humans, Inhibitory Concentration 50, Male, Oligosaccharides administration & dosage, Oligosaccharides pharmacology, Oligosaccharides therapeutic use, Plant Extracts administration & dosage, Plant Extracts therapeutic use, Sulfates, Testis enzymology, Enzyme Inhibitors pharmacology, Hyaluronoglucosaminidase antagonists & inhibitors, Phytotherapy, Plant Extracts pharmacology, Plants, Medicinal, Streptococcus agalactiae enzymology
- Abstract
Potent and specific inhibitors of hyaluronidases, a group of enzymes preferentially catalysing the hydrolysis of hyaluronic acid, are not known so far. Such compounds could be useful as pharmacological tools for studying the physiological and pathophysiological role of both hyaluronan and hyaluronidases. The effects of sulphated and non-sulphated structurally different oligosaccharides on bovine testicular hyaluronidase, hyaluronidase from bee venom and hyaluronate lyase from Streptococcus agalactiae (hylB (4755)) were studied with the Morgan-Elson reaction. Several active compounds were identified within a series of sulphated beta-(1,4)-galacto-oligosaccharides. The determined IC (50) values of these sulphated oligosaccharides ranged from 4 microM to 630 microM for all hyaluronan-degrading enzymes. Sulphated oligosaccharides like verbascose, planteose and neomycin showed comparable inhibition on all hyaluronidases, thereby possessing 100 - 500 times the activity of the widely accepted hyaluronidase inhibitor apigenin.
- Published
- 2005
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