Search

The present study was started with aim to design clotrimazole loaded polymeric microsphere based in situ ophthalmic gel for management of corneal fungal infections. The clotrimazole loaded microsphere was fabricated using various percentage of polylactide-co-glycolide polymer by solvent evaporation technique and evaluated for particle size, zeta potential and encapsulation efficiency. The polyvinyl alcohol was used as stabilizer. The formulation batch which showed good particle size and entrapment efficiency was selected for further study. The in situ ophthalmic gel of optimized drug loaded microsphere was formulated using various ratios of sodium alginate and hydroxy propyl methyl cellulose. The formulated gel was evaluated with respect to pH, in vitro gelling capacity, clarity, viscosity, in vitro antifungal activity and in vitro transcorneal permeation behavior. The fabricated drug loaded microspheres revealed acceptable particle size, zeta potential and encapsulation efficiency. The prepared in situ gels were clear and exhibited acceptable pH, rheological properties and in vitro gelation. The drug loaded microsphere based gel revealed superior in vitro antifungal activity against Aspergillus niger than conventional formulation and sufficient drug permeation across goat cornea. Thus, formulated clotrimazole loaded microsphere based in situ gel based systems can be a promising approach for sustained ophthalmic delivery of antifungal agents.

Ayurveda and Unani Medicine are the most classy and commonly practiced systems in India. In India’s AYUSH systems, some 8,000 herbal remedies have been codified. Amruthotharam/Amrutottaram Kashayam is one of such preparation which takes care of metabolic disorders through inflammation. The formulation contains guduchi (Tinospora cordifolia), haritaki (Terminalia chebula), and shunthi (Zingiber officinale) in the ratio 6:4:2. Natural remedies obtained from wild sources; therefore, sustaining consistent product quality is difficult because of extrinsic variables including soil conditions, light and water availability, temperature changes, nutrients, and geographic location. The present work aimed to develop and validate uni-dimensional double development high-performance thin layer chromatography to standardize the marker-based compounds such as gallic acid, berberine and gingerol-6, because the power of one-dimensional chromatography is often inadequate for complete resolution of the components present in complex samples which can be improved by separating actives through UDDD.

The different types of skin diseases caused due to microorganisms. In recent years the use of the traditional medicinal system was increased because of more minor side effects and cost effective. The single herbal drugs were found to be less potent, which can be improved by utilizing more than one herb in the single formulation, known as polyherbal formulation. The present work involved the development and evaluation of the different polyherbal formulations (cream, gel, and emulgel) using natural ingredients. The aim of the present work is to produce a formulation with improved antimicrobial potency and stability of formulations when compared with the individual extracts of herbal drugs. All the prepared formulations were tested against various microbial strains and concluded that the polyherbal formulations (C25, G1, EG1) were found potent against most selected strains. The prepared formulations can be used as a multipurpose formulation.

Physical harm that causes the skin to break or open up are known as wounds. For the repair of broken anatomical continuity and compromised functional status of the skin, proper wound healing is crucial. It is the end result of a coordinated response to harm from various cell types. The contraction and closure of the wound as well as the restoration of a functional barrier are the consequences of the intricate, multifactorial process known as wound healing. Due to its lower risk of side effects and lower cost, traditional medicine has seen increased use in recent years. When more than one herb is included in a single formulation, this is known as a polyherbal formulation, and the potency of the single herbal medications is increased. The purpose of the current study was to assess an emulgel made of developed polyherbal ingredients against wound healing. The current study aims to assess the created formulation’s ability to treat burns and excision wounds in terms of wound healing potential. Excise and burn wounds largely recovered. A group of rats given the medication showed signs of re-epithelialization of cells in newly formed tissue. At the healing site, there was also evidence of fibroblastic and vascular procreation. Without the presence of any microorganisms, the formulation effectively increases the rate of epithelialization and collagen viability across the wound region. According to the findings, the produced formulation (EG1) was superior to the extract in terms of effectiveness for wound healing.

Diabetes mellitus (DM) is a non-communicable disease that affects people all over the world and is defined by ongoing hyperglycemia. Sulfonylureas, biguanides, -glucosidase inhibitors, and non-sulfonylurea secretagogues are a few oral hypoglycemic medications that are frequently recommended by doctors for managing diabetes. Oral hypoglycemic medication use causes noticeable negative effects, and there is currently no permanent viable treatment for DM recovery. Complementary and alternative therapies must be used to lower the incidence of disease until better medical methods are discovered. The search for an efficient medication, either by itself or in combination, to treat diabetes continues to be fruitless. This might have a viable replacement in the shape of herbal preparations, which are widely employed in conventional medical systems. In order to determine the impact of Amruthotharam kashaya prepared using a traditional method for a four-week treatment period on blood glucose levels as well as other biochemical parameters like total cholesterol, LDL, HDL, and VLDL in HFD-alloxan-induced diabetic rats, the present study was designed. Significant weight loss was also seen with diabetes management, and this was partially reversed after formulation administration. The formulation significantly decreased increased levels of a few particular biochemical markers and avoided other hyperglycemia-related complications. These findings offer scientific support for the anti-diabetic usage of a conventional formulation and imply that the administration of the formulation to rats can be used safely by humans because it lowers the levels of several biochemical factors that contribute to diabetes.