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We studied the substrate enzyme activity, germination rate, seedling root length and seedling height of Brassica pekinensis seeds, Triticum aestivum seeds and Vigna radiate seeds, through the methanol extract the shell and embryo of Euscaphis japonica, with different original extract concentrations of 10%, 20%, 30% and 40% and extracted and separated the endogenous inhibitors in methanol leaching liquid of shell and embryo, the activity and ingredients of endogenous inhibitors in Euscaphis japonicaseeds. The results showed that with the increase of leaching solution concentration, the acid phosphatase activity and germination rate of Brassica pekinensis seeds significantly decreased(P<0.05), the inhibitory effects were showed increasing, and the inhibitory effects of shell were less than that of embryo; the root length and seedling heights promotion in low concentrations and inhibitory in high concentrations, and the maximum at the concentration of 10%, the promotion of shell less than embryo. The amylase activity, germination rate, root length and seedling height of Triticum aestivum seeds decreased gradually(P<0.05), the inhibitory effects were showed increasing gradually, while the germination rate was not significantly different at the concentration less than 20%(P>0.05)and it significantly decreased at 20% concentration(P<0.05), it was 0 at 40% concentration, the inhibitory effects of shell were than that of embryo. The protease activity, germination rate and seedling root length and shoot height of Vigna radiata seeds significantly decreased in the leaching solution concentration higher than 20%(P<0.05), and the effects of shell less than embryo. The results of extraction and separation of endogenous inhibitors showed that there were more phenolic acids and less alkaloids in shell than in embryo. It was concluded that high activating endogenous inhibitors existed in shell and embryo of Euscaphis japonica, which showed differences on properties, composition and content. Endogenous inhibitors in shell were mainly starchy materials, which in embryo mainly affected oil and protein materials. [ABSTRACT FROM AUTHOR]