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Objective: To establish an ultra-performance liquid chromatography quadrupole-time-of-flight mass spectrometric (UPLC-Q-TOF-MS) method for analyzing the flavonoids constituents in Apocynum venetom leaves., Methods: The analysis was performed on an Alltima C18 analytical column with a gradient solvent system of acetonitrile-1.0% acetic acid at the flow rate of 0.8 mL/min. The detection wavelength was at 254 nm and the column temperature was at 30 degrees C. Identification and detection were collected under the negative electrospray ion mode using Q-TOF-MS., Results: 11 flavonoids constituents being separated and identified, six of them were detected simultaneously. The results showed that the six detected flavonoids constituents had good linearity between concentrations and peak area (r > 0.9996), and the average recoveries of the method were between 94.3% - 103.3%., Conclusion: The method is accurate, reproducible, and can be used for the quality control of Apocynum venetom leaves.

Objective: To establish an HPLC method for simultaneous determination of four kinds of purines in deer fetus soft capsule., Methods: Four kinds of purines were detected and determined by HPLC. The mobile phase was 0.02 mol/L KH2PO4 (containing 1 mmol/L heptane sulfonic acid sodium, pH = 3.8)-methanol = 97:3. Detection wavelength was 254 nm and flow rate was 1.5 ml min. The linear relationship of four kinds of purines was as follows: hypoxanthine: Y1 = 83695X1 + 355 (r1 = 0.9998), with the linear range 0.040-0.667 mg/mL; xanthine: Y2 = 50638X2 + 39 (r2 = 0.9989), with the linear range 0.008-0.119 mg/mL; guanine: Y3 = 30269X3-9562 (r3 = 0.9924), with the linear range 0.018 - 0.279 mg/mL; adenine: Y4 = 38975X4-8671 (r4 = 0.9989), with the linear range 0.027-0.399 mg/mL The average sample recovery rate of hypoxanthine, xanthine, guanine and adenine were 98.1%, 98.6%, 98.0% and 97.5%, with RSD 1.0%, 0.4%, 0.8% and 0.6%, respectively., Results: The content of hypoxanthine, xanthine, guanine and adenine in 3 lots of deer fetus soft capsule were 116.5-132.0 microg/capsule, 21.2-23.0 microg/capsule, 48.6-54.3 microg/capsule and 68.9-75.2 microg/capsule, respectively., Conclusions: This method is simple,accurate and reproducible, which provides a basis for quality control of purines in deer fetus soft capsule.

Objective: To establish the analytical method for the fingerprint of Salviae Miltiorrhizae Radix et Rhizoma by HPCE-DAD and estimate its quality., Methods: Salviae Miltiorrhizae Radix et Rhizoma were analyzed and the chromatographic fingerprint were determined by HPCE-DAD. The data were analysed by fuzzy cluster and fingerprint similarity evaluation software was used to compare the similarity of samples., Results: HPCE-DAD fingerprint of 10 main common peaks was established preliminarily. It was discovered that a small number of samples were different from the others., Conclusion: The method is reliable, accurate and can be used for the quality control of Salviae Miltiorrhizae Radix et Rhizoma

Objective: To establish the analytical method for the fingerprint of Folium Apocyni Veneti by HPCE and estimate the quality of Folium Apocyni Veneti from different habitats., Methods: Folium Apocyni Veneti from different habitats were analyzed and the chromatographic fingerprint were determined by HPCE. The data were analysed by Fuzzy Cluster and Fingerprint Similarity Evaluation Software to compare the similarity of samples., Results: HPCE fingerprint of 7 main peaks was established preliminarily. It was discovered that a small number of samples differed from others., Conclusion: The method is reliable, accurate and can be used for quality control of Folium Apocyni Veneti.

Objective: To determine the dynamic change of essential components in Folium Apocyni Veneti, and provide scientific basis for determining the best collecting time., Methods: The essential components of Folium Apocyni Veneti from different harvesting time were analyzed by HSGC-MS. The relative content of the components were determined with peak area normalization method, and the dynamic changes of 7 main bioactive components were determined., Results: 39 constituents were separated and 38 compounds were identified. There were 21 co-containing compounds in Folium Apocyni Veneti from different harvesting time, the accumulation of which showed a regular pattern., Conclusion: The main essential components in Folium Apocyni Veneti are the highest in the traditional harvesting time.

Objective: To establish the analytical method for the fingerprint pattern of Cornu Elaphuri Davidiani by X-ray diffraction., Methods: Cornu Elaphuri Davidiani from different ages and different parts were analyzed and the fingerprint patterns were determined by X-ray diffraction. The degree of different fingerprint patterns was calculated and analyzed., Results: The reference X-ray diffraction Fourier fingerprint pattern and characteristic diffraction peaks of Cornu Elaphuri Davidiani were obtained by fifteen samples of Cornu Elaphuri Davidiani from different ages and different parts., Conclusion: This method can be used for identification and evaluation of Cornu Elaphuri Davidiani.

Objective: To study the identification of Herba Hyperici Japonici and its adulterant., Methods: Herba Hyperici Japonici and Herba Hyperici graminei were identified by traditional and modern identification., Results: Both of them showed difference in exterior character, microscope characteristic, TLC, UV and HPLC fingerprint graphics., Conclusion: The distinct characteristic features reveal this study can serve as evidence for the identification of Herba Hyperici Japonici.