1. [Study on UPLC Fingerprint of Corydalis bungeana].
- Author
-
Du YX, Zhai XT, Zhu FX, and Wang H
- Subjects
- Cluster Analysis, Drugs, Chinese Herbal, Principal Component Analysis, Chromatography, High Pressure Liquid, Corydalis chemistry, Ecosystem, Plants, Medicinal chemistry
- Abstract
Objective: To establish an Ultra Performance Liquid Chromatography fingerprint of Corydalis bungeana from different habitats., Methods: UPLC-PDA was adopted to analysis ten batches of Corydalis bungeana from different habitats with Phenomenex Luna Cāā column (250 mm x 4.6 mm, 5 µm) eluted with the mobile phase of acetonitrile and 0. 02% triethylamine in a gradient mode. The flow rate was 0.3 mL/min and the column temperature was 30 °C. The detection wavelength was set at 289 nm., Results: The fingerprints of ten batches of Corydalis bungeana from different habitats had 13 common peaks, three of them were identified. The similarities were larger than 0.80. Ten batches of samples were divided into three categories by cluster analysis. Three principal components were ob- served via principal component analysis and the value of three principal components accounted for 89. 607% of the total variance. Two major chemical components of Corydalis bungeana were confirmed., Conclusion: The high-performance and rapid method is successfully used for fingerprint analysis and can be used to evaluate the quality of Corydalis bungeana.
- Published
- 2015