1. A laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva
- Author
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Fourniol, Lisa, Madec, Yoann, Mousson, Laurence, Vazeille, Marie, Failloux, Anna-Bella, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology, Pasteur-Cnam Risques infectieux et émergents (PACRI), Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), This study was supported by the European Union’s Horizon 2020 research and innovation program under EVAg grant agreement no. 653316 and the French Government’s Investissement d’Avenir program, Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' (grant n°ANR-10-LABX-62-IBEID)., We thank Malika Hocine and Jocelyne Alexandre for helping in rearing mosquitoes., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 653316,H2020,H2020-INFRAIA-2014-2015,EVAg(2015), Institut Pasteur [Paris], and Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)
- Subjects
RNA viruses ,Physiology ,viruses ,[SDV]Life Sciences [q-bio] ,Disease Vectors ,Pathology and Laboratory Medicine ,Mosquitoes ,Medical Conditions ,Filter Paper ,Animal Products ,Aedes ,Medicine and Health Sciences ,Chikungunya Virus ,Reverse Transcriptase Polymerase Chain Reaction ,Eukaryota ,virus diseases ,Agriculture ,Honey ,Body Fluids ,Insects ,Laboratory Equipment ,Infectious Diseases ,Blood ,Medical Microbiology ,Viral Pathogens ,Viruses ,Engineering and Technology ,RNA, Viral ,Medicine ,Pathogens ,Anatomy ,Salivation ,Research Article ,Paper ,Arthropoda ,Alphaviruses ,Science ,education ,Equipment ,Aedes Aegypti ,Microbiology ,Specimen Handling ,Togaviruses ,parasitic diseases ,Animals ,Saliva ,Microbial Pathogens ,Nutrition ,fungi ,Organisms ,Biology and Life Sciences ,Invertebrates ,Insect Vectors ,Diet ,Species Interactions ,Food ,Physiological Processes ,Zoology ,Entomology - Abstract
International audience; Mosquito control is implemented when arboviruses are detected in patients or in field-collected mosquitoes. However, mass screening of mosquitoes is usually laborious and expensive, requiring specialized expertise and equipment. Detection of virus in mosquito saliva using honey-impregnated filter papers seems to be a promising method as it is non-destructive and allows monitoring the viral excretion dynamics over time from the same mosquito. Here we test the use of filter papers to detect chikungunya virus in mosquito saliva in laboratory conditions, before proposing this method in large-scale mosquito surveillance programs. We found that 0.9 cm 2 cards impregnated with a 50% honey solution could replace the forced salivation technique as they offered a viral RNA detection until 7 days after oral infection of Aedes aegypti and Aedes albopictus mosquitoes with CHIKV.
- Published
- 2021
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