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2 results on '"Aditya Nayak"'

1. Impact of process and physico-chemical conditions on the formation of curcumin-whey protein composite particles capable to stabilize food-compatible oil in water emulsions

Author

Anne Meynier, Angélina Dorlando, Isabelle Capron, Claude Genot, Aditya Nayak, TCI Bangalore, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), INRAE Pays de la Loire, and European Project: 609398,EC:FP7:PEOPLE,FP7-PEOPLE-2013-COFUND,AGREENSKILLSPLUS(2014)

Subjects
Whey protein, Flocculation, Curcumin, Chemistry, Pickering stabilization, Composite nanoparticles, Dispersity, Composite number, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, [CHIM.GENI]Chemical Sciences/Chemical engineering, Chemical engineering, Oil-in-water emulsion, Ionic strength, Emulsion, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Particle, Surface charge, 0210 nano-technology, human activities, Food Science
Abstract

International audience; Use of nanonized curcumin-whey protein (CWP) composite particles as Pickering stabilizers are an attractive strategy due to their edibility and natural origin. This study aimed to examine the effect of physico-chemical conditions (pH, ionic strength, protein form) during the formation of CWP composite nanoparticles on their physico-chemical characteristics (size, surface charge, morphology) and further their Pickering ability by influencing their aptitude to stabilize oil/water interfaces. Our study demonstrated that, to favor the protein-curcumin interactions and to improve the particle characteristics (smaller size, monodispersity), one should increase the accessibility of hydrophobic moiety of proteins (denaturation) and control ionic strength. Increasing the concentration of the CWP composite nanoparticles decreased size and polydispersity enhancing emulsion stability. Use of these CWP composite nanoparticles in food products with high ionic strength will tend to flocculation and thereby emulsions destabilization. This work allows for a better understanding of the role of physico-chemical conditions on the formation of CWP composite nanoparticles and thereof their oil/water interfacial activity.

Published
2022

2. A circRNA from SEPALLATA3 regulates splicing of its cognate mRNA through R-loop formation

Author

Chloe Zubieta, Markus Schmid, Vanessa M. Conn, Aditya Nayak, Véronique Hugouvieux, Agnès Jourdain, Simon J. Conn, Gökhan Cildir, Giovanna Capovilla, Vinay Tergaonkar, Stephanie A. Conos, Centre for Cancer Biology, Hanson Institute, Adelaide, Physiologie cellulaire et végétale (LPCV), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Department of Molecular Biology [Tübingen], Max Planck Institute for Developmental Biology, Max-Planck-Gesellschaft-Max-Planck-Gesellschaft, Department of Biochemistry, Yong Loo Lin School of Medicine and Department of Biological Sciences, National University of Singapore (NUS), Laboratory of NF-κB Signaling, Institute of Molecular and Cell Biology, Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, ANR (FLOPINET), CNRS, NHMRC [GNT1089167], Australian Research Council [FT160100318], ATIP-Avenir, LabEx GRAL [ANR-10-LABX-49-01], South Australian Government Department of State Development, CEA Irtelis fellowship, ANR-16-CE92-0023,FLOPINET,Floral Pioneer Factors(2016), ANR-10-LABX-0049,GRAL,Grenoble Alliance for Integrated Structural Cell Biology(2010), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Conn, Vanessa M, Hugouvieux, Véronique, Nayak, Aditya, Conos, Stephanie A, Capovilla, Giovanna, Cildir, Gökhan, Jourdain, Agnés, Tergaonkar, Vinay, Schmid, Markus, Zubieta, Chloe, Conn, Simon J, Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), ANR-16-CE92-0023,FLOPINET,Floral Pioneer Factors, and ANR-10-LABX-49-01,Labex GRAL

Subjects
0106 biological sciences, 0301 basic medicine, circular RNAs (circRNAs), DNA, Plant, RNA Splicing, SEP3, Arabidopsis, Plant Science, Biology, 01 natural sciences, Flowering, 03 medical and health sciences, Splicing factor, Exon, alternative splicing, Circular RNA, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, RNA, Messenger, Gene, SEPALLATA3, Homeodomain Proteins, Genetics, Arabidopsis Proteins, Alternative splicing, RNA, Plant, Gene regulation, 030104 developmental biology, back-splicing, RNA splicing, DNA, Circular, Transcription factor, Homeotic gene, Transcription Factors, 010606 plant biology & botany
Abstract

Simon J. Conn (simon.conn@unisa.edu.au); International audience; Circular RNAs (circRNAs) are a diverse and abundant class of hyper-stable, non-canonical RNAs that arise through a form of alternative splicing (AS) called back-splicing. These single-stranded, covalently-closed circRNA molecules have been identified in all eukaryotic kingdoms of life(1), yet their functions have remained elusive. Here, we report that circRNAs can be used as $bona\ fide$ biomarkers of functional, exon-skipped AS variants in $Arabidopsis$, including in the homeotic MADS-box transcription factor family. Furthermore, we demonstrate that circRNAs derived from exon 6 of the SEPALLATA3 (SEP3) gene increase abundance of the cognate exon-skipped AS variant (SEP3.3 which lacks exon 6), in turn driving floral homeotic phenotypes. Toward demonstrating the underlying mechanism, we show that the SEP3 exon 6 circRNA can bind strongly to its cognate DNA locus, forming an RNA:DNA hybrid, or R-loop, whereas the linear RNA equivalent bound significantly more weakly to DNA. R-loop formation results in transcriptional pausing, which has been shown to coincide with splicing factor recruitment and AS(2-4). This report presents a novel mechanistic insight for how at least a subset of circRNAs probably contribute to increased splicing efficiency of their cognate exon-skipped messenger RNA and provides the first evidence of an organismal-level phenotype mediated by circRNA manipulation.

Published
2017

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