1. Ectopic expression of dehydration responsive element binding proteins (StDREB2) confers higher tolerance to salt stress in potato
- Author
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Donia Bouaziz, Hela Ben Amor, Mondher Bouzayen, Mariam Charfeddine, Julien Pirrello, Radhia Gargouri-Bouzid, Asma Hammami, Amina Dhieb, Laboratoire des Biotechnologies Végétales Appliquées à l'Amélioration des Cultures (Sfax, Tunisie), Université de Sfax - University of Sfax, Génomique et Biotechnologie des Fruits (GBF), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure Agronomique de Toulouse-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Institut National de la Recherche Agronomique (INRA)-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National Polytechnique (Toulouse) (Toulouse INP), Tunisian Ministry of High Education and Scientific Research, Institut National Polytechnique de Toulouse - INPT (FRANCE), Institut National de la Recherche Agronomique - INRA (FRANCE), Université de Sfax (TUNISIA), and Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
- Subjects
Chlorophyll ,0106 biological sciences ,[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,Physiology ,DREB ,Plant Science ,Genetically modified crops ,Plant Roots ,01 natural sciences ,chemistry.chemical_compound ,Plant Growth Regulators ,Gene Expression Regulation, Plant ,[INFO.INFO-BT]Computer Science [cs]/Biotechnology ,Abscisic acid ,Phylogeny ,Plant Proteins ,2. Zero hunger ,Regulation of gene expression ,Abiotic component ,0303 health sciences ,Dehydration ,Ornithine-Oxo-Acid Transaminase ,food and beverages ,Salt Tolerance ,Plants, Genetically Modified ,Cell biology ,Cold Temperature ,Organ Specificity ,Protein Binding ,Signal Transduction ,Proline ,Génomique, Transcriptomique et Protéomique ,Molecular Sequence Data ,Biotechnologies ,Abiotic stresses ,Biology ,03 medical and health sciences ,Stress, Physiological ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Botany ,Genetics ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Electrophoretic mobility shift assay ,Amino Acid Sequence ,Transcription factor ,030304 developmental biology ,Solanum tuberosum ,fungi ,Transgenic plants ,Plant Components, Aerial ,chemistry ,Ectopic expression ,Sequence Alignment ,Biologie végétale ,Abscisic Acid ,Transcription Factors ,010606 plant biology & botany - Abstract
Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; International audience; Dehydration responsive element binding proteins (DREB) are members of a larger family of transcription factors, many of which have been reported to contribute to plant responses to abiotic stresses in several species. While, little is known about their role in potato (Solanum tuberosum). This report describes the cloning and characterization of a DREB transcription factor cDNA, StDREB2, isolated from potato(cv Nicola) plants submitted to salt treatment. Based on a multiple sequence alignment, this protein was classified into the A-5 group of DREB subfamily. Expression studies revealed that StDREB2 was induced in leaves, roots and stems upon various abiotic stresses and in response to exogenous treatment with abscisic acid (ABA). In agreement with this expression pattern, over-expression of StDREB2 in transgenic potato plants resulted in enhanced tolerance to salt stress. These data suggest that the isolated StDREB2 encodes a functional protein involved in plant response to different abiotic stresses. An electrophoretic mobility shift assay (EMSA) indicated that the StDREB2 protein bound specifically to the DRE core element (ACCGAGA) in vitro. Moreover, Semi quantitative RT-PCR analysis revealed that the transcript level of a putative target gene i.e. d1-pyrroline-5-carboxylate synthase (P5CS) was upregulated in transgenic plants submitted to salt stress conditions. A concomitant increase in proline accumulation was also observed under these conditions. Taking together, all these data suggest that StDREB2 takes part in the processes underlying plant responses to abiotic stresses probably via the regulation of ABA hormone signaling and through a mechanism allowing proline synthesis.
- Published
- 2012