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2 results on '"CRABS-CLAW"'

1. A Light-Regulated Genetic Module Was Recruited to Carpel Development in Arabidopsis following a Structural Change to SPATULA

Author

Françoise Monéger, Aurélie Chauvet, Mathieu C. Reymond, Jaime F. Martínez-García, Géraldine Brunoud, Marie-Laure Martin-Magniette, Charles P. Scutt, Reproduction et développement des plantes (RDP), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon), Univ Autonoma Barcelona, Consejo Super Invest Cient, Inst Recerca & Tecnol Agroalimentaries,Inst Catal, Universitat de Barcelona (UB), Unité de recherche en génomique végétale (URGV), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Mathématiques et Informatique Appliquées (MIA-Paris), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Genoplante and the French National Research Agency [ANR-BLAN-0211-01], Spanish Ministerio de Economia, Hacienda o Finanzas, European Regional Development Fund [BIO2011-23489], Rhone-Alpes doctoral studentship, Agence Nationale de la Recherche (France), Ministerio de Ciencia e Innovación (España), European Commission, Région Auvergne-Rhône-Alpes, Ministerio de Economía y Competitividad (España), École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Recherche Agronomique (INRA), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), and Institut National de la Recherche Agronomique (INRA)-AgroParisTech

Subjects
0106 biological sciences, Models, Molecular, FRUIT-DEVELOPMENT, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Light, Arabidopsis, Plant Science, 01 natural sciences, CRABS-CLAW, Gene Expression Regulation, Plant, Phytochrome B, Basic Helix-Loop-Helix Transcription Factors, Arabidopsis thaliana, Promoter Regions, Genetic, Phylogeny, Research Articles, 0303 health sciences, biology, Phytochrome, TRANSCRIPTION FACTOR SPATULA, food and beverages, Gene Expression Regulation, Developmental, Plants, Genetically Modified, Cell biology, Phenotype, Flowering plant, SHADE-AVOIDANCE-RESPONSE, Gynoecium, Molecular Sequence Data, SIGNAL-TRANSDUCTION, Flowers, BHLH PROTEIN, Evolution, Molecular, GYNOECIUM, 03 medical and health sciences, Shade avoidance, Botany, TARGET GENES, Amino Acid Sequence, Nucleotide Motifs, Transcription factor, 030304 developmental biology, Key innovation, RED-LIGHT, Base Sequence, THALIANA, Arabidopsis Proteins, fungi, Cell Biology, Sequence Analysis, DNA, biology.organism_classification, Protein Structure, Tertiary, Mutation, Transcriptome, 010606 plant biology & botany
Abstract

A key innovation of flowering plants is the female reproductive organ, the carpel. Here, we show that a mechanism that regulates carpel margin development in the model flowering plant Arabidopsis thaliana was recruited from light-regulated processes. This recruitment followed the loss from the basic helix-loop-helix transcription factor SPATULA (SPT) of a domain previously responsible for its negative regulation by phytochrome. We propose that the loss of this domain was a prerequisite for the light-independent expression in female reproductive tissues of a genetic module that also promotes shade avoidance responses in vegetative organs. Striking evidence for this proposition is provided by the restoration of wild-type carpel development to spt mutants by low red/far-red light ratios, simulating vegetation shade, which we show to occur via phytochrome B, PHYTOCHROME INTERACTING FACTOR4 (PIF4), and PIF5. Our data illustrate the potential of modular evolutionary events to generate rapid morphological change and thereby provide a molecular basis for neo-Darwinian theories that describe this nongradualist phenomenon. Furthermore, the effects shown here of light quality perception on carpel development lead us to speculate on the potential role of light-regulated mechanisms in plant organs that, like the carpel, form within the shade of surrounding tissues., We acknowledge funding from Génoplante and the French National Research Agency (ANR-BLAN-0211-01) to C.P.S., Spanish Ministerio de Economía, Hacienda o Finanzas and European Regional Development Fund (BIO2011-23489) funding to J.F.M.-G., and a Rhône-Alpes doctoral studentship to M.C.R.

Published
2012
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2. Down-regulation of a single auxin efflux transport protein in tomato induces precocious fruit development

Author

Virginie Garcia, Catherine Deborde, Mariusz Kowalczyk, Koh Aoki, Antonio Granell, Martine Lemaire-Chamley, Catherine Bellini, Alisdair R. Fernie, Johann Petit, Fabien Mounet, Mickaël Maucourt, Kentaro Yano, Johannes Rohrmann, Hélène Bergès, Christophe Rothan, Annick Moing, Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Department of Plant Physiology, Umea Plant Science Centre, Umeå University-Umeå University, Centre National de Ressources Génomiques Végétales (CNRGV), Institut National de la Recherche Agronomique (INRA), Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Region Aquitaine [20051303006ABC], EUSOL [FOOD-CT-2006-016214], Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA)-Université Bordeaux Segalen - Bordeaux 2, Umea Plant Science Center (UPSC), Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences (SLU)-Swedish University of Agricultural Sciences (SLU), COGNITIQUE, Laboratoire de l'intégration, du matériau au système (IMS), Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Université Sciences et Technologies - Bordeaux 1, Meiji University, Kazusa DNA Research Institute (KDRI), Universitat Politècnica de València (UPV), and Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)

Subjects
0106 biological sciences, Auxin efflux, Physiology, [SDV]Life Sciences [q-bio], Plant Science, Parthenocarpy, Plant Roots, 01 natural sciences, CRABS-CLAW, Solanum lycopersicum, Plant Growth Regulators, Gene Expression Regulation, Plant, Biologiska vetenskaper, PIN proteins, Phylogeny, ComputingMilieux_MISCELLANEOUS, transcription factor, Oligonucleotide Array Sequence Analysis, Plant Proteins, Regulator gene, chemistry.chemical_classification, 0303 health sciences, Bud, Auxin efflux transport protein (PIN), Gene Expression Regulation, Developmental, food and beverages, Biological Sciences, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, Plants, Genetically Modified, Up-Regulation, Cell biology, Phenotype, RNA Interference, fruit set, tomato (Solanum lycopersicum), Research Paper, Signal Transduction, Down-Regulation, Flowers, Biology, 03 medical and health sciences, Auxin, Botany, MADS-BOX, 030304 developmental biology, Indoleacetic Acids, Auxin homeostasis, Gene Expression Profiling, fungi, Biological Transport, biology.organism_classification, chemistry, Fruit, parthenocarpy, Solanum, Transcription Factors, 010606 plant biology & botany
Abstract

Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; The PIN-FORMED (PIN) auxin efflux transport protein family has been well characterized in the model plant Arabidopsis thaliana, where these proteins are crucial for auxin regulation of various aspects of plant development. Recent evidence indicates that PIN proteins may play a role in fruit set and early fruit development in tomato (Solanum lycopersicum), but functional analyses of PIN-silenced plants failed to corroborate this hypothesis. Here it is demonstrated that silencing specifically the tomato SlPIN4 gene, which is predominantly expressed in tomato flower bud and young developing fruit, leads to parthenocarpic fruits due to precocious fruit development before fertilization. This phenotype was associated with only slight modifications of auxin homeostasis at early stages of flower bud development and with minor alterations of ARF and Aux/IAA gene expression. However, microarray transcriptome analysis and large-scale quantitative RT-PCR profiling of transcription factors in developing flower bud and fruit highlighted differentially expressed regulatory genes, which are potential targets for auxin control of fruit set and development in tomato. In conclusion, this work provides clear evidence that the tomato PIN protein SlPIN4 plays a major role in auxin regulation of tomato fruit set, possibly by preventing precocious fruit development in the absence of pollination, and further gives new insights into the target genes involved in fruit set.

Published
2012
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