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3 results on '"Dirk Lindemann"'

1. Identification of an Intermediate Step in Foamy Virus Fusion

Author

Aurélie Dupont, Ivo M. Glück, Dorothee Ponti, Kristin Stirnnagel, Sylvia Hütter, Florian Perrotton, Nicole Stanke, Stefanie Richter, Dirk Lindemann, Don C. Lamb, Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] (LIPhy ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Ludwig-Maximilians-Universität München (LMU), and Technische Universität Dresden = Dresden University of Technology (TU Dresden)

Subjects
Life Cycle Stages, foamy virus, single virus tracking, viruses, [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], lcsh:QR1-502, Virion, envelope glycoprotein, Virus Internalization, Virus Replication, Membrane Fusion, Models, Biological, lcsh:Microbiology, Article, retrovirus, fluorescence live cell imaging, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], capsid, Humans, Spumavirus, viral fusion, spinning disk confocal microscopy, Retroviridae Infections
Abstract

Viral glycoprotein-mediated membrane fusion is an essential step for productive infection of host cells by enveloped viruses, however, due to its rarity and challenges in detection, little is known about the details of fusion events at the single particle level. Here, we have developed dual-color foamy viruses (FVs) composed of eGFP-tagged prototype FV (PFV) Gag and mCherry-tagged Env of either PFV or macaque simian FV (SFVmac) origin that have been optimized for detection of the fusion process. Using our recently developed tracking imaging correlation (TrIC) analysis, we were able to detect the fusion process for both PFV and SFVmac Env containing virions. PFV Env-mediated fusion was observed both at the plasma membrane as well as from endosomes, whereas SFVmac Env-mediated fusion was only observed from endosomes. PFV Env-mediated fusion was observed to happen more often and more rapidly than as for SFVmac Env. Strikingly, using the TrIC method, we detected a novel intermediate state where the envelope and capsids are still tethered but separated by up to 400 nm before final separation of Env and Gag occurred.

Published
2020

2. Ortervirales: New Virus Order Unifying Five Families of Reverse-Transcribing Viruses

Author

Katja R. Richert-Pöggeler, Emmanuelle Muller, Neil E. Olszewski, Michael Tristem, Pierre-Yves Teycheney, Benham E Lockhart, Balázs Harrach, Hanu R. Pappu, Jens Mayer, Andrew D. W. Geering, Jan Kreuze, John M. Coffin, Robert J. Gifford, Jonathan P. Stoye, James E. Schoelz, Hung Fan, Mikhail M. Pooggin, Livia Stavolone, Susan Seal, Hélène Sanfaçon, Sead Sabanadzovic, Jens H. Kuhn, Carlos Llorens, Welkin E. Johnson, Eugene V. Koonin, Dirk Lindemann, Indranil Dasgupta, Mart Krupovic, Jonas Blomberg, Roger Hull, Biologie Moléculaire du Gène chez les Extrêmophiles (BMGE), Institut Pasteur [Paris], Uppsala University, Tufts University School of Medicine [Boston], University of Delhi, University of California [Irvine] (UCI), University of California, University of Queensland [Brisbane], University of Glasgow, Hungarian Academy of Sciences (MTA), John Innes Centre [Norwich], Boston College (BC), International Potato Center [Lima] (CIP), Consultative Group on International Agricultural Research [CGIAR] (CGIAR), Institute of Virology [Dresden], Technische Universität Dresden = Dresden University of Technology (TU Dresden), Universitat de València (UV), University of Minnesota [Twin Cities] (UMN), University of Minnesota System, Saarland University [Saarbrücken], Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Washington State University (WSU), Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Julius Kühn-Institut - Federal Research Centre for Cultivated Plants (JKI), Mississippi State University [Mississippi], Agriculture and Agri-Food [Ottawa] (AAFC), University of Missouri [Columbia] (Mizzou), University of Missouri System, University of Greenwich, International Institute of Tropical Agriculture, Imperial College London, National Institutes of Health [Bethesda] (NIH), This work was supported in part through Battelle Memorial Institute's prime contract with the U.S. National Institute of Allergy and Infectious Diseases (NIAID, contract no. HHSN272200700016I, J.H.K.). E.V.K. is supported by intramural funds from the U.S. Department of Health and Human Services (to the National Library of Medicine). S.S. acknowledges support from SRI Funds from Mississippi Agriculture and the Forestry Experiment Station of Mississippi State University. J.F.K. is supported by the CGIAR Research Program on Roots, Tubers and Bananas (RTB) and supported by CGIAR Fund Donors (http://www.cgiar.org/aboutus/our-funders/). M.K. is supported by l’Agence Nationale de la Recherche (France) project ENVIRA., M. Krupovic, B. Harrach, S. Sabanadzovic, H. Sanfaçon, and J. H. Kuhn were members of the 2014–2017 International Committee on Taxonomy of Viruses (ICTV) Executive Committee. J. Blomberg, J. M. Coffin, H. Fan, R. Gifford, W. Johnson, D. Lindemann, J. Mayer, J. P. Stoye, and M. Tristem were members of the 2014–2017 ICTV Retroviridae Study Group. I. Dasgupta, A. D. Geering, R. Hull, J. F. Kreuze, B. Lockhart, E. Muller, N. Olszewski, H. R. Pappu, M. Pooggin, K. R. Richert-Pöggeler, J. E. Schoelz, S. Seal, L. Stavolone, and P.-Y. Teycheney were members of the 2014–2017 ICTV Caulimoviridae Study Group. R. Hull is retired from the John Innes Centre, Norwich, Norfolk, United Kingdom, ANR-17-CE15-0005,ENVIRA,Remodelage de la membrane cytoplasmique par les virus enveloppés d'archées(2017), International Potato Center, Technische Universität Dresden (TUD), University of Minnesota [Twin Cities], Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Julius Kühn Institute (JKI), University of Missouri [Columbia], Institut Pasteur [Paris] (IP), University of California [Irvine] (UC Irvine), University of California (UC), Biotechnology and Biological Sciences Research Council (BBSRC), Agriculture and Agri-Food (AAFC), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)

Subjects
0301 basic medicine, S1, retroviruses, viruses, [SDV]Life Sciences [q-bio], Immunology, retroviridae, MESH: Reverse Transcription, L73 - Maladies des animaux, Virus Replication, [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, Microbiology, Virus, belpaoviridae, MESH: Viruses, 03 medical and health sciences, Virology, international committee on taxonomy of viruses (ICTV), Metaviridae, virus classification, Letter to the Editor, Virus classification, Genetics, Ty3/Gypsy and Ty1/Copia LTR retrotransposons, caulimoviridae, virus evolution, biology, fungi, MESH: Virus Replication, RNA, Pseudoviridae, Reverse Transcription, biology.organism_classification, MESH: Caulimoviridae, genomic DNA, 030104 developmental biology, MESH: Retroviridae, MESH: Hepadnaviridae, Insect Science, Viral evolution, hepadnaviridae, Belpaoviridae, Caulimoviridae, Hepadnaviridae, International Committee on Taxonomy of Viruses (ICTV), Retroviridae, Viruses, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, metaviridae, pseudoviridae
Abstract

International audience; Reverse-transcribing viruses, which synthesize a copy of genomic DNA from an RNA template, are widespread in animals, plants, algae, and fungi (1, 2). This broad distribution suggests the ancient origin(s) of these viruses, possibly [...]

Published
2018

3. The cooperative function of arginine residues in the Prototype Foamy Virus Gag C-terminus mediates viral and cellular RNA encapsidation

Author

Juliane Reh, Erik Müllers, Nicole Stanke, Winfried Weissenhorn, Dirk Lindemann, Martin V. Hamann, Grégory Effantin, Institut de biologie structurale (IBS - UMR 5075 ), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Unit for Virus Host-Cell Interactions [Grenoble] (UVHCI), Centre National de la Recherche Scientifique (CNRS)-European Molecular Biology Laboratory [Grenoble] (EMBL)-Université Joseph Fourier - Grenoble 1 (UJF), Thomas, Frank, Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), and Université Joseph Fourier - Grenoble 1 (UJF)-European Molecular Biology Laboratory [Grenoble] (EMBL)-Centre National de la Recherche Scientifique (CNRS)

Subjects
RNA packaging, [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], viruses, [SDV]Life Sciences [q-bio], Assembly, Mutation, Missense, Gene Products, gag, Arginine, Genome, Virus, Cell Line, Protein structure, Virology, Humans, Spumavirus, Sequence Deletion, Gag, biology, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], C-terminus, Virus Assembly, Research, RNA, biology.organism_classification, Reverse transcriptase, [SDV] Life Sciences [q-bio], Infectious Diseases, Capsid, Amino Acid Substitution, Foamy virus
Abstract

Background One unique feature of the foamy virus (FV) capsid protein Gag is the absence of Cys-His motifs, which in orthoretroviruses are irreplaceable for multitude functions including viral RNA genome recognition and packaging. Instead, FV Gag contains glycine-arginine-rich (GR) sequences at its C-terminus. In case of prototype FV (PFV) these are historically grouped in three boxes, which have been shown to play essential functions in genome reverse transcription, virion infectivity and particle morphogenesis. Additional functions for RNA packaging and Pol encapsidation were suggested, but have not been conclusively addressed. Results Here we show that released wild type PFV particles, like orthoretroviruses, contain various cellular RNAs in addition to viral genome. Unlike orthoretroviruses, the content of selected cellular RNAs in capsids of PFV vector particles was not altered by viral genome encapsidation. Deletion of individual GR boxes had only minor negative effects (2 to 4-fold) on viral and cellular RNA encapsidation over a wide range of cellular Gag to viral genome ratios examined. Only the concurrent deletion of all three PFV Gag GR boxes, or the substitution of multiple arginine residues residing in the C-terminal GR box region by alanine, abolished both viral and cellular RNA encapsidation (>50 to >3,000-fold reduced), independent of the viral production system used. Consequently, those mutants also lacked detectable amounts of encapsidated Pol and were non-infectious. In contrast, particle release was reduced to a much lower extent (3 to 20-fold). Conclusions Taken together, our data provides the first identification of a full-length PFV Gag mutant devoid in genome packaging and the first report of cellular RNA encapsidation into PFV particles. Our results suggest that the cooperative action of C-terminal clustered positively charged residues, present in all FV Gag proteins, is the main viral protein determinant for viral and cellular RNA encapsidation. The viral genome independent efficiency of cellular RNA encapsidation suggests differential packaging mechanisms for both types of RNAs. Finally, this study indicates that analogous to orthoretroviruses, Gag – nucleic acid interactions are required for FV capsid assembly and efficient particle release. Electronic supplementary material The online version of this article (doi:10.1186/s12977-014-0087-7) contains supplementary material, which is available to authorized users.

Published
2014

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