1. Soluble HLA-G Expression Inversely Correlates With Fetal Microchimerism Levels in Peripheral Blood From Women With Scleroderma
- Author
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Julie Di Cristofaro, Karlin R. Karlmark, Sami B. Kanaan, Doua F. Azzouz, Marina El Haddad, Lucas Hubert, Dominique Farge-Bancel, Brigitte Granel, Jean Robert Harlé, Eric Hachulla, Etienne Pardoux, Jean Roudier, Christophe Picard, Nathalie C. Lambert, Anthropologie bio-culturelle, Droit, Ethique et Santé (ADES), Aix Marseille Université (AMU)-EFS ALPES MEDITERRANEE-Centre National de la Recherche Scientifique (CNRS), Arthrites autoimmunes (AA), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), EFS ALPES MEDITERRANEE, Département d'Oncologie (Dep Oncol - AVIGNON), Institut Ste Catherine, Centre recherche en CardioVasculaire et Nutrition (C2VN), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Assistance Publique-Hôpitaux de Marseille (AP-HM), Service de médecine interne [Lille], Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Institut de Mathématiques de Marseille (I2M), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Assistance Publique - Hôpitaux de Marseille (APHM), Dubois Frid, Caroline, and Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU)
- Subjects
0301 basic medicine ,Male ,systemic sclerosis ,[SDV]Life Sciences [q-bio] ,Gene Expression ,Fetal Development ,0302 clinical medicine ,Gene Frequency ,Untranslated Regions ,HLA-G ,Genotype ,Immunology and Allergy ,scleroderma ,ComputingMilieux_MISCELLANEOUS ,Original Research ,Microchimerism ,Middle Aged ,fetal ,[SDV] Life Sciences [q-bio] ,Female ,pregnancy ,lcsh:Immunologic diseases. Allergy ,Adult ,medicine.medical_specialty ,Offspring ,Immunology ,Human leukocyte antigen ,Chimerism ,03 medical and health sciences ,Internal medicine ,medicine ,microchimerism ,Humans ,Alleles ,Aged ,Autoantibodies ,HLA-G Antigens ,Fetus ,Pregnancy ,Polymorphism, Genetic ,Scleroderma, Systemic ,business.industry ,Autoantibody ,medicine.disease ,030104 developmental biology ,Endocrinology ,Haplotypes ,human leukocyte antigen-G ,Case-Control Studies ,lcsh:RC581-607 ,business ,030215 immunology - Abstract
International audience; Women with scleroderma (SSc) maintain significantly higher quantities of persisting fetal microchimerism (FMc) from complete or incomplete pregnancies in their peripheral blood compared to healthy women. The non-classical class-I human leukocyte antigen (HLA) molecule HLA-G plays a pivotal role for the implantation and maintenance of pregnancy and has often been investigated in offspring from women with pregnancy complications. However data show that maternal HLA-G polymorphisms as well as maternal soluble HLA-G (sHLA-G) expression could influence pregnancy outcome. Here, we aimed to investigate the underlying role of maternal sHLA-G expression and HLA-G polymorphisms on the persistence of FMc. We measured sHLA-G levels by enzyme linked immunosorbent assay in plasma samples from 88 healthy women and 74 women with SSc. Male Mc was quantified by DYS14 real-time PCR in blood samples from 58 women who had previously given birth to at least one male child. Furthermore, eight HLA-G 5'URR/3'UTR polymorphisms, previously described as influencing HLA-G expression, were performed on DNA samples from 96 healthy women and 106 women with SSc. Peripheral sHLA-G was at lower concentration in plasma from SSc (76.2 ± 48.3 IU/mL) compared to healthy women (117.5 ± 60.1 IU/mL, p
- Published
- 2018