1. Sperm Vitality and Necrozoospermia: Diagnosis, Management, and Results of a Global Survey of Clinical Practice
- Author
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Yoshiharu Morimoto, Kasonde Bowa, Israel Maldonado Rosas, Marjan Sabbaghian, Ralf Henkel, Juan Manuel Corral Molina, Chak-Lam Cho, Kristian Leisegang, Hisanori Taniguchi, Mohamed Ali Sadighi Gilani, Germar-Michael Pinggera, Sunil Jindal, Christopher Chee Kong Ho, Gianmaria Salvio, Marlon Martinez, Juan Carlos Alvarez, Taha A. Abdel-Meguid, Jean de la Rosette, Taymour Mostafa, Mohamed S. Al-Marhoon, Sava Micic, Ashok Agarwal, Jonathan Ramsay, Samantha B. Schon, Ala'a Farkouh, Kareim Khalafalla, Gian Maria Busetto, Nicholas N. Tadros, Sajal Gupta, Edoardo Pescatori, Gökhan Çeker, Renata Finelli, Ayad Palani, Damayanthi Durairajanayagam, Marcelo Gabriel Rodriguez, Florence Boitrelle, Rupin Shah, Eric Chung, Mohamed Arafa, Ramadan A Saleh, Christina Anagnostopoulou, Shinnosuke Kuroda, Parviz Kavoussi, Shinichiro Fukuhara, Armand Zini, Giovanni M. Colpi, Paraskevi Vogiatzi, Neel Parekh, Hussein Kandil, Tan V. Le, Raghavender Kosgi, Ponco Birowo, Rossella Cannarella, Giancarlo Balercia, Mara Simopoulou, Sijo Parekattil, Ettore Caroppo, Aldo E. Calogero, Mesut Berkan Duran, Osvaldo Rajmil, Amarnath Rambhatla, Haitham Elbardisi, Hyun Jun Park, Saleem Ali Banihani, Fotios Dimitriadis, Edmund Y. Ko, Rakesh Sharma, Cleveland Clinic, CHI Poissy-Saint-Germain, Biologie de la Reproduction, Environnement, Epigénétique & Développement (BREED), École nationale vétérinaire d'Alfort (ENVA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Hamad Medical Corporation [Doha, Qatar], Weill Cornell Medicine [Qatar], The Chinese University of Hong Kong [Hong Kong], Henry Ford Health System, Imperial College London, University of the Western Cape, The University of Texas at San Antonio (UTSA), Loma Linda University, Fakih IVF Fertility Center, University of Garmian, Università Politecnica delle Marche [Ancona] (UNIVPM), Cairo University - Faculty of Medicine, Fundació Puigvert [Barcelona, Spain], Jordan University of Science and Technology [Irbid, Jordan], University of Michigan Medical School [Ann Arbor], University of Michigan [Ann Arbor], University of Michigan System-University of Michigan System, Pham Ngoc Thach Medical University [Ho Chi Minh city, Vietnam] (PNTUM), Faculty of Medicine [Jakarta, Indonesia], Universitas Indonesia [Jakarta, Indonesia], Taylor's University Malaysia, University of Catania [Italy], Hamad medical corporation, McGill University = Université McGill [Montréal, Canada], University of Queensland [Brisbane], Aristotle University of Thessaloniki, Innsbruck Medical University [Austria] (IMU), Università degli Studi di Foggia - University of Foggia, Kansai Medical University, Pusan National University, Hammersmith Hospital NHS Imperial College Healthcare, National and Kapodistrian University of Athens (NKUA), Royan Institute for Reproductive Biomedicine [Tehran, Iran], UNIVERSITY OF SANTO TOMAS MANILA PHL, Partenaires IRSTEA, Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), Sultan Qaboos University (SQU), Università degli studi di Catania [Catania], Osaka University [Osaka], University of Central Florida [Orlando] (UCF), Minia University, King Abdulaziz University, and Lilavati Hospital and Research Centre
- Subjects
Aging ,Vitality ,0302 clinical medicine ,Pharmacology (medical) ,TESTICULAR SPERMATOZOA ,Andrology ,Sperm motility ,0303 health sciences ,030219 obstetrics & reproductive medicine ,Spermatozoon ,Health Policy ,Urology & Nephrology ,Spermatozoa ,3. Good health ,Psychiatry and Mental health ,medicine.anatomical_structure ,Asthenozoospermia ,EosineYellowish-(YS) ,Eosine Yellowish-(YS) ,Life Sciences & Biomedicine ,Infertility ,medicine.medical_specialty ,endocrine system ,Urology ,Semen ,ICSI ,PARAMETERS ,03 medical and health sciences ,Endocrinology & Metabolism ,medicine ,Intensive care medicine ,030304 developmental biology ,Nigrosin ,Science & Technology ,SEMINAL PLASMA ,business.industry ,urogenital system ,MALE-INFERTILITY ,Public Health, Environmental and Occupational Health ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,medicine.disease ,Sperm ,VIABILITY ,IMMOTILE ,Health Care Sciences & Services ,Reproductive Medicine ,DNA-DAMAGE ,SEMEN QUALITY ,Etiology ,business ,HYPOOSMOTIC SWELLING TEST - Abstract
International audience; Sperm vitality testing is a basic semen examination that has been described in the World Health Organization (WHO) Laboratory Manual for the Examination and Processing of Human Semen from its primary edition, 40 years ago. Several methods can be used to test sperm vitality, such as the eosin-nigrosin (E-N) stain or the hypoosmotic swelling (HOS) test. In the 6th (2021) edition of the WHO Laboratory Manual, sperm vitality assessment is mainly recommended if the total motility is less than 40%. Hence, a motile spermatozoon is considered alive, however, in certain conditions an immotile spermatozoon can also be alive. Therefore, the differentiation between asthenozoospermia (pathological decrease in sperm motility) and necrozoospermia (pathological decrease in sperm vitality) is important in directing further investigation and management of infertile patients. The causes leading to necrozoospermia are diverse and can either be local or general, testicular or extra-testicular. The andrological management of necrozoospermia depends on its etiology. However, there is no standardized treatment available presently and practice varies among clinicians. In this study, we report the results of a global survey to understand current practices regarding the physician order of sperm vitality tests as well as the management practices for necrozoospermia. Laboratory and clinical scenarios are presented to guide the reader in the management of necrozoospermia with the overall objective of establishing a benchmark ranging from the diagnosis of necrozoospermia by sperm vitality testing to its clinical management.
- Published
- 2021