1. Elucidating Sequence and Structural Determinants of Carbohydrate Esterases for Complete Deacetylation of Substituted Xylans
- Author
-
Leena Penttinen, Vera Kouhi, Régis Fauré, Tatiana Skarina, Peter Stogios, Emma Master, Edita Jurak, University of Eastern Finland, Helsingin yliopisto = Helsingfors universitet = University of Helsinki, Toulouse Biotechnology Institute (TBI), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Toronto, Department of Bioproducts and Biosystems, Aalto University, University of Groningen [Groningen], KSLA Tandem Forest Value projectTFV2018-0009, European Project: 648925,H2020,ERC-2014-CoG,BHIVE(2015), University of Helsinki, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés, University of Groningen, Aalto-yliopisto, and Bioproduct Engineering
- Subjects
Acetyl xylan esterase ,Carbohydrate esterase ,acetyl xylan esterase ,Organic Chemistry ,carbohydrate esterase ,Esterases ,Pharmaceutical Science ,Acetates ,SGNH Hydrolase ,xylan ,Substrate Specificity ,Analytical Chemistry ,Xylan ,Chemistry (miscellaneous) ,Drug Discovery ,Molecular Medicine ,Xylans ,Physical and Theoretical Chemistry ,SGNH hydrolase ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] - Abstract
openaire: EC/H2020/648925/EU//BHIVE Acetylated glucuronoxylan is one of the most common types of hemicellulose in nature. The structure is formed by a β-(1→4)-linked D-xylopyranosyl (Xylp) backbone that can be substituted with an acetyl group at O-2 and O-3 positions, and α-(1→2)-linked 4-O-methylglucopyranosyluronic acid (MeGlcpA). Acetyl xylan esterases (AcXE) that target mono-or doubly acetylated Xylp are well characterized; however, the previously studied AcXE from Flavobacterium johnsoniae (FjoAcXE) was the first to remove the acetyl group from 2-O-MeGlcpA-3-O-acetyl-substituted Xylp units, yet structural characteristics of these enzymes remain unspecified. Here, six homologs of FjoAcXE were produced and three crystal structures of the enzymes were solved. Two of them are complex structures, one with bound MeGlcpA and another with acetate. All homologs were confirmed to release acetate from 2-O-MeGlcpA-3-O-acetyl-substituted xylan, and the crystal structures point to key structural elements that might serve as defining features of this unclassified carbohydrate esterase family. Enzymes comprised two domains: N-terminal CBM domain and a C-terminal SGNH domain. In FjoAcXE and all studied homologs, the sequence motif around the catalytic serine is Gly-Asn-Ser-Ile (GNSI), which differs from other SGNH hydrolases. Binding by the MeGlcpA-Xylp ligand is directed by positively charged and highly conserved residues at the interface of the CBM and SGNH domains of the enzyme.
- Published
- 2022