1. Susceptibility of polar cod (Boreogadus saida) to a model carcinogen
- Author
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Svetlana A. Murzina, Jasmine Nahrgang, Maura Benedetti, Adélaïde Lerebours, Francesco Regoli, Knut Erik Tollefsen, Jeanette M. Rotchell, You Song, Université de La Rochelle (ULR), LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs), Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS), School of Biological Sciences, University of Portsmouth, Karelian Research Center of the Russian Academy of Science (KarRC RAS ), Norwegian Institute for Water Research (NIVA), Polytechnic University of Marche [Ancona, Italy], University of Hull [United Kingdom], and The Arctic University of Norway (UiT)
- Subjects
Boreogadus saida ,DNA repair ,Carcinogenesis ,Spleen ,010501 environmental sciences ,Aquatic Science ,Biology ,Oceanography ,01 natural sciences ,Andrology ,DNA Adducts ,03 medical and health sciences ,chemistry.chemical_compound ,[SDV.EE.ECO]Life Sciences [q-bio]/Ecology, environment/Ecosystems ,DNA adduct ,medicine ,Animals ,Bile ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] ,Carcinogen ,ComputingMilieux_MISCELLANEOUS ,030304 developmental biology ,0105 earth and related environmental sciences ,[SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health ,0303 health sciences ,cancer genes ,DNA repair genes ,polar cod ,General Medicine ,biology.organism_classification ,Pollution ,Gadiformes ,Gonadosomatic Index ,medicine.anatomical_structure ,Benzo(a)pyrene ,chemistry ,Micronucleus test ,Carcinogens ,benzo(a)pyrene ,[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology - Abstract
Studies that aim to characterise the susceptibility of the ecologically relevant and non-model fish polar cod (Boreogadus saida) to model carcinogens are required. Polar cod were exposed under laboratory conditions for six months to control, 0.03 μg BaP/g fish/week and 0.3 μg BaP/g fish/week dietary benzo(a)pyrene (BaP), a reference carcinogen. The concentrations of the 3-OH-BaP bile metabolite and transcriptional responses of genes involved in DNA adduct recognition (xpc), helicase activity (xpd), DNA repair (xpf, rad51) and tumour suppression (tp53) were assessed after 0, 1, 3 and 6 months of exposure, alongside body condition indexes (gonadosomatic index, hepatosomatic index and condition factor). Micronuclei and nuclear abnormalities in blood and spleen, and liver histopathological endpoints were assessed at the end of the experiment. Fish grew steadily over the whole experiment and no mortality was recorded. The concentrations of 3-OH-BaP increased significantly after 1 month of exposure to the highest BaP concentration and after 6 months of exposure to all BaP concentrations showing the biotransformation of the mother compound. Nevertheless, no significant induction of gene transcripts involved in DNA damage repair or tumour suppression were observed at the selected sampling times. These results together with the absence of chromosomal damage in blood and spleen cells, the subtle increase in nuclear abnormalities observed in spleen cells and the low occurrence of foci of cellular alteration suggested that the exposure was below the threshold of observable effects. Taken together, the results showed that polar cod was not susceptible to carcinogenesis using the BaP exposure regime employed herein.
- Published
- 2021
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