1. Quality control of commercial cranberry products: HPTLC-densitometry a new deal
- Author
-
Alain Gueiffier, Joëlle Dorat, Frank Bruyère, Arnaud Lanoue, Leslie Boudesocque-Delaye, Cécile Enguehard-Gueiffier, Infectiologie Santé Publique (ISP-311), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Biomolécules et biotechnologies végétales (BBV EA 2106), Université de Tours, Nutrition, croissance et cancer (U 1069) (N2C), Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Francois Rabelais [Tours], Université de Tours-Institut National de la Recherche Agronomique (INRA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Tours, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Université de Tours (UT), and Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)
- Subjects
0301 basic medicine ,CUPLC-MS ,media_common.quotation_subject ,01 natural sciences ,03 medical and health sciences ,Ingredient ,[CHIM.ANAL]Chemical Sciences/Analytical chemistry ,[SDV.IDA]Life Sciences [q-bio]/Food engineering ,Vaccinium macrocarpon ,Sample preparation ,Quality (business) ,Cranberry ,media_common ,030109 nutrition & dietetics ,Chromatography ,Chemistry ,010401 analytical chemistry ,Quality control ,0104 chemical sciences ,Proanthocyanidin ,Polyphenol ,Uplc ms ms ,HPTL ,Densitometry ,Food Science ,Biotechnology - Abstract
International audience; A fast quality control protocol based on reference BL-DMAC assay and High-performance thin-layer chromatography (HPTLC)-Densitometry was optimized for routine quality control of cranberry (Vaccinium macrocarpon) products. The sample preparation appeared as a key step to ensure a relevant comparison of products quality and biological data. Sequential solid/liquid extractions were required to fully extract polyphenols, regardless the extraction solvent chosen. The HPTLC-densitometry herein protocol used epicatechin, PAC-A2 and PAC-B2 as quality markers and densitometric measurements at 200 nm. The PAC-A2/Epicatechin ratio, obtained using HPTLC, appeared as an appropriate indicator of the quality of cranberry ingredient. Only two products, among the tested panel, exhibited a high quality cranberry-based ingredient using this criteria. UPLC-MS analyses coupled to multivariate analyses confirmed HPTLC-densitometry conclusions. This work highlighted the plural PAC composition of commercial products and the lack of standardization for cranberry-based products on the market, explaining the non-reproducibility of clinical trials.
- Published
- 2018
- Full Text
- View/download PDF