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International audience; We analyzed 21 rockfalls that occurred in limestone cliffs of the Chartreuse Massif (French Alps). These rockfalls were detected both by Terrestrial Laser Scanning or photogrammetry and by a local seismological network. The combination of these methods allowed us to study relations between rockfall properties (location of detachment and impacts areas, volume, geometry, propagation) and the induced seismic signal. We observed events with different propagation modes (sliding, mass flow, free-fall) that could be determined from Digital Elevation Models. We focused on events that experienced a free-fall after their detachment. We analyzed the first parts of the seismic signals corresponding to the detachment phase and to the first impact. The detachment phase has a smaller amplitude than the impact phase, and its amplitude and duration increasewith rockfall volume. By measuring the time delay between the detachment phase and the first impact, we can estimate the free-fall height. We found a relation Es=aEpbbetween the potential energy of a rockfall Ep and the seismic energy Esgenerated during the first impact, with parameters a=10-8and b=1.55 and with a correlation coefficient R²=0.98. We can thus estimate both the potential energy of a block and its free-fall height from the seismic signals. By combining these results, we obtain an accurate estimate of the rockfall volume. This relation was then tested on different geological settings and for larger volumes using Yosemite and Mount Granier rockfalls. We also compared our results with a data set of controlled releases of single blocks (Hibert et al., 2017) in order to investigate smaller volumes.

Background Understanding the effects of resistance QTL on pathogen development cycle is an important issue for the creation of QTL combination strategies to durably increase disease resistance in plants. The oomycete pathogen Aphanomyces euteiches, causing root rot disease, is one of the major factors limiting the pea crop in the main producing countries. No commercial resistant varieties are currently available in Europe. Resistance alleles at seven main QTL were recently identified and introgressed into pea agronomic lines, resulting in the creation of Near Isogenic Lines (NILs) at the QTL. This study aimed to determine the effect of main A. euteiches resistance QTL in NILs on different steps of the pathogen life cycle. Results NILs carrying resistance alleles at main QTL in susceptible genetic backgrounds were evaluated in a destructive test under controlled conditions. The development of root rot disease severity and pathogen DNA levels in the roots was measured during ten days after inoculation. Significant effects of several resistance alleles at the two major QTL Ae-Ps7.6 and Ae-Ps4.5 were observed on symptom appearance and root colonization by A. euteiches. Some resistance alleles at three other minor-effect QTL (Ae-Ps2.2, Ae-Ps3.1 and Ae-Ps5.1) significantly decreased root colonization. The combination of resistance alleles at two or three QTL including the major QTL Ae-Ps7.6 (Ae-Ps5.1/Ae-Ps7.6 or Ae-Ps2.2/Ae-Ps3.1/Ae-Ps7.6) had an increased effect on delaying symptom appearance and/or slowing down root colonization by A. euteiches and on plant resistance levels, compared to the effects of individual or no resistance alleles. Conclusions This study demonstrated the effects of single or multiple resistance QTL on delaying symptom appearance and/or slowing down colonization by A. euteiches in pea roots, using original plant material and a precise pathogen quantification method. Our findings suggest that single resistance QTL can act on multiple or specific steps of the disease development cycle and that their actions could be pyramided to increase partial resistance in future pea varieties. Further studies are needed to investigate QTL effects on different steps of the pathogen life cycle, as well as the efficiency and durability of pyramiding strategies using QTL which appear to act on the same stage of the pathogen cycle. Electronic supplementary material The online version of this article (doi:10.1186/s12870-016-0822-4) contains supplementary material, which is available to authorized users.