1. HSPCs display within-family homogeneity in differentiation and proliferation despite population heterogeneity
- Author
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Ken R. Duffy, Tamar Tak, Leïla Perié, Caroline Marty, Gaël Simon, Camélia Benlabiod, Isabelle Plo, Giulio Prevedello, Noémie Paillon, Institut Curie [Paris], Laboratoire Physico-Chimie Curie [Institut Curie] (PCC), Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Centre de Formation et de Recherche sur les Environnements Méditérranéens (CEFREM), Université de Perpignan Via Domitia (UPVD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Gustave Roussy (IGR), Dynamique moléculaire de la transformation hématopoïétique (Dynamo), Institut Gustave Roussy (IGR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, Hamilton Institute [NUI], National University of Ireland [Galway] (NUI Galway), and Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0301 basic medicine ,concordance ,Cell type ,Mouse ,QH301-705.5 ,progenitor cell ,Cellular differentiation ,Science ,[SDV]Life Sciences [q-bio] ,Bone Marrow Cells ,Biology ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Bone Marrow ,medicine ,Animals ,Progenitor cell ,Biology (General) ,Cells, Cultured ,Ancestor ,[PHYS]Physics [physics] ,General Immunology and Microbiology ,Cluster of differentiation ,General Neuroscience ,Cell Differentiation ,General Medicine ,medicine.disease ,Hematopoietic Stem Cells ,Stem Cells and Regenerative Medicine ,Mice, Inbred C57BL ,Haematopoiesis ,Leukemia ,030104 developmental biology ,cell proliferation ,Evolutionary biology ,Medicine ,haematopoietic stem ,Stem cell ,030217 neurology & neurosurgery ,Research Article - Abstract
International audience; High-throughput single-cell methods have uncovered substantial heterogeneity in the pool of hematopoietic stem and progenitor cells (HSPCs), but how much instruction is inherited by offspring from their heterogeneous ancestors remains unanswered. Using a method that enables simultaneous determination of common ancestor, division number, and differentiation status of a large collection of single cells, our data revealed that murine cells that derived from a common ancestor had significant similarities in their division progression and differentiation outcomes. Although each family diversifies, the overall collection of cell types observed is composed of homogeneous families. Heterogeneity between families could be explained, in part, by differences in ancestral expression of cell surface markers. Our analyses demonstrate that fate decisions of cells are largely inherited from ancestor cells, indicating the importance of common ancestor effects. These results may have ramifications for bone marrow transplantation and leukemia, where substantial heterogeneity in HSPC behavior is observed.
- Published
- 2021
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