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3 results on '"Istituto Nazionale Genetica Molecolare [Milano] (INGM)"'

1. Fatty acid metabolism complements glycolysis in the selective regulatory T cell expansion during tumor growth

Author

Giuseppe Matarese, Nico Mitro, Massimiliano Pagani, Martina Severa, Chiara Focaccetti, Yu Wei, Eliana M. Coccia, Stefano Miacci, Silvia Piconese, Eleonora Timperi, Claudio Procaccini, Giuseppe Danilo Norata, Ilenia Pacella, Valeria Ranzani, Vincenzo Barnaba, Fabiana Rizzo, Grazisa Rossetti, Fabrizia Bonacina, Ezio Giorda, Deriggio Faicchia, Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome] (UNIROMA), Consiglio Nazionale delle Ricerche [Napoli] (CNR), Istituto Superiore di Sanità (ISS), Università degli Studi di Milano = University of Milan (UNIMI), Curtin University [Perth], Planning and Transport Research Centre (PATREC), Istituto Nazionale Genetica Molecolare [Milano] (INGM), Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Pathogenèse des Virus de l'Hépatite B (PVHB), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), University of Naples Federico II = Università degli studi di Napoli Federico II, Institut Pasteur, Fondation Cenci Bolognetti - Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Réseau International des Instituts Pasteur (RIIP), Istituto Italiano di Tecnologia (IIT), This work was supported by Associazione Italiana per la Ricerca sul Cancro Grants IG-2014 15199 and IG-2017 19939 (to V.B.) and IG-2017 19784 (to S.P.), Ministry of Education, University and Research (MIUR) Grants RF-2010-2310438 and RF 2010-2318269, Fondazione Italiana Sclerosi Multipla Grants code 2015/R/04 (to V.B.), code 2013/R/9 (to E.M.C.), code 2016/R/18 (to G.M.), and code 2014/R/19 (to M.S.), MIUR Progetti di Ricerca di Interesse Nazionale Grant 2010-2011 protocol 2010LC747T_004, MIUR Fondo per gli Investimenti della Ricerca di Base 2011/13 Grant no. RBAP10TPXK, Istituto Pasteur Italia–Fondazione Cenci Bolognetti Grant 2014-2016, International Network Institut Pasteur Programmes Transversaux de Recherche Grant 20-16, Fondazione Roma Grant for Biomedical Research NCDS-2013-000000345, the European Union’s Seventh Framework Program (FP7) under Grant Agreement 259743 (MODHEP consortium) (to Y.W.), Fondazione Cariplo Grant 2016-0852 (to G.D.N.), Ministero della Salute Grants GR-2011-02346974 (to G.D.N.) and GR-2013-02355011 (to F.B.), and European Union IDEAS Programme European Research Council Starting Grant menTORingTregs 310496 and Telethon Grant GGP17086 (to G.M.). C.F. was supported by a 2015 Fellowship from Fondazione Veronesi., European Project: 259743,EC:FP7:HEALTH,FP7-HEALTH-2010-two-stage,MODHEP(2011), European Project: 310496,EC:FP7:ERC,ERC-2012-StG_20111109,MENTORINGTREGS(2013), Pacella, Ilenia, Procaccini, Claudio, Focaccetti, Chiara, Miacci, Stefano, Timperi, Eleonora, Faicchia, Deriggio, Severa, Martina, Rizzo, Fabiana, Coccia, Eliana Marina, Bonacina, Fabrizia, Mitro, Nico, Norata, Giuseppe Danilo, Rossetti, Grazisa, Ranzani, Valeria, Pagani, Massimiliano, Giorda, Ezio, Wei, Yu, Matarese, Giuseppe, Barnaba, Vincenzo, Piconese, Silvia, Istituto Superiore di Sanita [Rome], Systems biology of liver cancer: an integrative genomic-epigenomic approach - MODHEP - - EC:FP7:HEALTH2011-01-01 - 2016-06-30 - 259743 - VALID, Unravelling paradoxes in regulatory T cell biology: the molecular basis for an mTOR-dependent oscillatory metabolic switch controlling immune tolerance and autoimmunity - MENTORINGTREGS - - EC:FP7:ERC2013-05-01 - 2018-04-30 - 310496 - VALID, Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], Università degli Studi di Milano [Milano] (UNIMI), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), and Università degli studi di Napoli Federico II

Subjects
0301 basic medicine, MESH: Neoplasm Proteins, MESH: Oxidation-Reduction, Treg, fatty acid synthesis, glycolysis, ox40, tumor microenvironment, Glycolysi, T-Lymphocytes, Type I, Settore MED/04, T-Lymphocytes, Regulatory, Transgenic, chemistry.chemical_compound, Mice, 0302 clinical medicine, Neoplasms, MESH: Tumor Microenvironment, Glycolysis, MESH: Animals, Tumor, Multidisciplinary, Chemistry, Fatty Acids, hemic and immune systems, Regulatory, Cell biology, Neoplasm Proteins, MESH: Fatty Acids, Fatty Acid Synthase, Type I, medicine.anatomical_structure, MESH: Neoplasms, Experimental, PNAS Plus, Fatty Acid Synthase, 030220 oncology & carcinogenesis, MESH: Glycolysis, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH: Fatty Acid Synthase, Type I, Fatty acid synthesis, Ox40, Tumor microenvironment, Animals, Cell Line, Tumor, Humans, Mice, Transgenic, Neoplasms, Experimental, Oxidation-Reduction, Tumor Microenvironment, Intracellular, MESH: Cell Line, Tumor, [SDV.IMM] Life Sciences [q-bio]/Immunology, Regulatory T cell, MESH: Mice, Transgenic, T cell, chemical and pharmacologic phenomena, Cell Line, 03 medical and health sciences, Experimental, Fatty acid synthesi, medicine, MESH: Mice, MESH: Humans, Fatty acid metabolism, MESH: T-Lymphocytes, Regulatory, Lipid metabolism, 030104 developmental biology
Abstract

International audience; The tumor microenvironment restrains conventional T cell (Tconv) activation while facilitating the expansion of Tregs. Here we showed that Tregs' advantage in the tumor milieu relies on supplemental energetic routes involving lipid metabolism. In murine models, tumor-infiltrating Tregs displayed intracellular lipid accumulation, which was attributable to an increased rate of fatty acid (FA) synthesis. Since the relative advantage in glucose uptake may fuel FA synthesis in intratumoral Tregs, we demonstrated that both glycolytic and oxidative metabolism contribute to Tregs' expansion. We corroborated our data in human tumors showing that Tregs displayed a gene signature oriented toward glycolysis and lipid synthesis. Our data support a model in which signals from the tumor microenvironment induce a circuitry of glycolysis, FA synthesis, and oxidation that confers a preferential proliferative advantage to Tregs, whose targeting might represent a strategy for cancer treatment.

Published
2018
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2. Guidelines for Developing Successful Short Advanced Courses in Systems Medicine and Systems Biology

Author

Francesco Marabita, David Gomez-Cabrero, Jesper Tegnér, Josep Roca, Ana Conesa, Philippe Sabatier, Isaac Cano, Sonia Tarazona, Integrative Biology Program [Milano], Istituto Nazionale Genetica Molecolare [Milano] (INGM), Centro de Genómica - Centre de Genòmica [IVIA], Instituto Valenciano de Investigaciones Agrarias - Institut Valencià d'Investigacions Agraries - Valencian Institute for agricultural Research (IVIA), Environnement et Prédiction de la Santé des Populations (TIMC-IMAG-EPSP), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Computational Biology Unit, Linköping University (LIU), and ZOPPIS, Catherine

Subjects
0301 basic medicine, Health Knowledge, Attitudes, Practice, Engineering, Systems Analysis, Histology, Knowledge management, Systems biology, [SDV]Life Sciences [q-bio], ESTADISTICA E INVESTIGACION OPERATIVA, MEDLINE, Interdisciplinary Studies, Nature versus nurture, Education, Pathology and Forensic Medicine, 03 medical and health sciences, 0302 clinical medicine, ComputingMilieux_COMPUTERSANDEDUCATION, Humans, Students, Curriculum, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, business.industry, Systems Biology, 4. Education, Interdisciplinary learning, Cell Biology, Research Personnel, 3. Good health, [SDV] Life Sciences [q-bio], Systems medicine, 030104 developmental biology, Systems analysis, Engineering ethics, business, 030217 neurology & neurosurgery
Abstract

[EN] Systems medicine and systems biology have inherent educational challenges. These have largely been addressed either by providing new masters programs or by redesigning undergraduate programs. In contrast, short courses can respond to a different need: they can provide condensed updates for professionals across academia, the clinic, and industry. These courses have received less attention. Here, we share our experiences in developing and providing such courses to current and future leaders in systems biology and systems medicine. We present guidelines for how to reproduce our courses, and we offer suggestions for how to select students who will nurture an interdisciplinary learning environment and thrive there., The authors would like to acknowledge helpful comments from the editor. J.T. was supported by EU FP7 305033 CASyM and King Abdullah University for Science and Technology. D.G., F.M., S.T, A.C., and J.T. were supported by EU FP7 306000 STATegra

Published
2017

3. Purification of LAT containing membranes from resting and activated T lymphocytes

Author

Claire Hivroz, Paola Larghi, Mabel Juana Jouve San Roman, Laurence Ardouin, Hivroz, Claire, Immunité et cancer (U932), Université Paris Descartes - Paris 5 (UPD5)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Università degli Studi di Milano = University of Milan (UNIMI), Istituto Nazionale Genetica Molecolare [Milano] (INGM), and University of Milan

Subjects
[SDV] Life Sciences [q-bio], [SDV.IMM] Life Sciences [q-bio]/Immunology, [SDV]Life Sciences [q-bio], [SDV.IMM]Life Sciences [q-bio]/Immunology
Abstract

International audience; In T lymphocytes, the immune synapse is an active zone of vesicular traffic. Directional transport of vesicular receptors and signaling molecules from or to the immune synapse has been shown to play an important role in T-cell receptor (TCR) signal transduction. However, how vesicular trafficking is regulating the activation of T cells is still a burning question, and the characterization of these intracellular compartments remains the first step to understand this process. We describe herein a protocol, which combines a separation of membranes on flotation gradient with an affinity purification of Strep-tagged fusion transmembrane proteins with Strep-Tactin® resin, allowing the purification of membranes containing the Strep-tagged molecule of interest. By keeping the membranes intact, this protocol leads to the purification of molecules physically associated with the Strep-tagged protein as well as of molecules present in the same membrane compartment: transmembrane proteins, proteins strongly associated with the membranes, and luminal proteins. The example shown herein is the purification of membrane compartment prepared from T lymphocytes expressing LAT fused to a Strep-tag.

Published
2017

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