1. Cell Wall Proteome of Wheat Grain Endosperm and Outer Layers at Two Key Stages of Early Development
- Author
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Rogniaux Hélène, Geairon Audrey, Larré Colette, Bouder Axelle, Cherkaoui Mehdi, Jamet Elisabeth, Guillon Fabienne, Lollier Virginie, Francin-Allami Mathilde, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire de Recherche en Sciences Végétales (LRSV), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), INRA (National Institute of Agronomic Research), Centre National de la Recherche Scientifique (CNRS), European Commission, and Paul Sabatier-Toulouse 3 University.
- Subjects
0106 biological sciences ,0301 basic medicine ,Signal peptide ,Cellular differentiation ,proteome ,[SDV]Life Sciences [q-bio] ,01 natural sciences ,Catalysis ,Endosperm ,lcsh:Chemistry ,Inorganic Chemistry ,Cell wall ,03 medical and health sciences ,wheat ,Compartment (development) ,Physical and Theoretical Chemistry ,grain ,lcsh:QH301-705.5 ,Molecular Biology ,Spectroscopy ,remodeling ,2. Zero hunger ,Chemistry ,Organic Chemistry ,food and beverages ,General Medicine ,Computer Science Applications ,Cell biology ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,polysaccharide ,Proteome ,cell wall ,Cellularization ,Esterase inhibitor ,010606 plant biology & botany - Abstract
International audience; The cell wall is an important compartment in grain cells that fulfills both structural and functional roles. It has a dynamic structure that is constantly modified during development and in response to biotic and abiotic stresses. Non-structural cell wall proteins (CWPs) are key players in the remodeling of the cell wall during events that punctuate the plant life. Here, a subcellular and quantitative proteomic approach was carried out to identify CWPs possibly involved in changes in cell wall metabolism at two key stages of wheat grain development: the end of the cellularization step and the beginning of storage accumulation. Endosperm and outer layers of wheat grain were analyzed separately as they have different origins (maternal and seed) and functions in grains. Altogether, 734 proteins with predicted signal peptides were identified (CWPs). Functional annotation of CWPs pointed out a large number of proteins potentially involved in cell wall polysaccharide remodeling. In the grain outer layers, numerous proteins involved in cutin formation or lignin polymerization were found, while an unexpected abundance of proteins annotated as plant invertase/pectin methyl esterase inhibitors were identified in the endosperm. In addition, numerous CWPs were accumulating in the endosperm at the grain filling stage, thus revealing strong metabolic activities in the cell wall during endosperm cell differentiation, while protein accumulation was more intense at the earlier stage of development in outer layers. Altogether, our work gives important information on cell wall metabolism during early grain development in both parts of the grain, namely the endosperm and outer layers. The wheat cell wall proteome is the largest cell wall proteome of a monocot species found so far.
- Published
- 2020
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