1. Staphylococcus aureus Decreases SUMOylation Host Response to Promote Intramacrophage Survival
- Author
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Nadhuma Youssouf, Virginie Molle, Philippe Soubeyran, Clara Recasens-Zorzo, Guillaume Bossis, Laila Gannoun-Zaki, LPHI - Laboratory of Pathogen Host Interactions (LPHI), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Centre de Recherche en Cancérologie de Marseille (CRCM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Aix Marseille Université (AMU), Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), MOLLE, Virginie, and Laboratory of Pathogen Host Interactions [Montpellier] (LPHI)
- Subjects
Staphylococcus aureus ,QH301-705.5 ,intracellular survival ,Host response ,SUMO protein ,macrophage ,medicine.disease_cause ,Catalysis ,Microbiology ,Inorganic Chemistry ,03 medical and health sciences ,Ubc9 ,medicine ,Macrophage ,Biology (General) ,Physical and Theoretical Chemistry ,QD1-999 ,Molecular Biology ,Spectroscopy ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,biology ,Host (biology) ,Brief Report ,030302 biochemistry & molecular biology ,Organic Chemistry ,General Medicine ,biology.organism_classification ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,SUMOylation ,infection ,3. Good health ,Computer Science Applications ,Chemistry ,Enzyme ,chemistry ,[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Bacteria ,Intracellular - Abstract
Staphylococcus aureus is a commensal bacterium that causes severe infections in soft tissue and the bloodstream. During infection, S. aureus manipulates host cell response to facilitate its own replication and dissemination. Here, we show that S. aureus significantly decreases the level of SUMOylation, an essential post-translational modification, in infected macrophages 24 h post-phagocytosis. The reduced level of SUMOylation correlates with a decrease in the SUMO-conjugating enzyme Ubc9. The over-expression of SUMO proteins in macrophages impaired bacterial intracellular proliferation and the inhibition of SUMOylation with ML-792 increased it. Together, these findings demonstrated for the first time the role of host SUMOylation response toward S. aureus infection.
- Published
- 2021
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