1. SPIX: a new software package to reveal chemical reactions at trace amounts in very complex mixtures from high-resolution mass spectra data sets
- Author
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Nicol, Edith, Xu, Yao, Varga, Zsuzsanna, Kinani, Said, Bouchonnet, Stéphane, Lavielle, Marc, Laboratoire de chimie moléculaire (LCM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X), Modélisation en pharmacologie de population (XPOP), Centre de Mathématiques Appliquées - Ecole Polytechnique (CMAP), École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS)-Inria Saclay - Ile de France, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Laboratoire National d’Hydraulique et Environnement (EDF R&D LNHE), EDF R&D (EDF R&D), EDF (EDF)-EDF (EDF), Lavielle, Marc, and École polytechnique (X)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
High-resolution mass spectrometry ,complex mixtures ,kinetics ,[MATH.MATH-ST]Mathematics [math]/Statistics [math.ST] ,[CHIM] Chemical Sciences ,software development ,spectral big data management ,chemical reactions ,[CHIM]Chemical Sciences ,non-targeted approach ,[MATH.MATH-ST] Mathematics [math]/Statistics [math.ST] - Abstract
International audience; Rationale: High-resolution mass spectrometry-based non-targeted screening has a huge potential for applications in environmental sciences, engineering and regulation. However, it produces big data for which full appropriate processing is a real challenge; the development of processing software is the last building-block to enable large-scale use of this approach. Methods: A new software application, SPIX, has been developed to extract relevant information from high-resolution mass-spectrum datasets. Dealing with intrinsic sample variability and reducing operator subjectivity, it opens up opportunities and promising prospects in many areas of analytical chemistry. SPIX is freely available at: http://spix.webpopix.org. Results: Two features of the software are presented in the field of environmental analysis. An example illustrates how SPIX reveals photodegradation reactions in wastewater by fitting kinetic models to significant changes in ion abundance over time. A second example shows the ability of SPIX to detect photoproducts at trace amounts in river water, through comparison of datasets from samples taken before and after irradiation. Conclusions: SPIX has shown its ability to reveal relevant modifications between two series of big data sets, allowing for instance to study the consequences of a given event on a complex substrate. Most of alland this is to our knowledge the only software currently available allowing thatit can reveal and monitor any kind of reaction in all types of mixture.
- Published
- 2020