1. Influence of incubation time on antimicrobial susceptibility testing of pathogenic Vibrio anguillarum and Vibrio vulnificus isolated from fish
- Author
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Lone Madsen, Kári Karbech Mouritsen, Sophie A. Granier, Pete Smith, Joop Testerink, Sandrine Baron, Eva Blomkvist, Daniela Ceccarelli, Thierry Morin, Isabelle Kempf, Michal A Voorbergen-Laarman, Eric Jouy, Kees Veldman, Emeline Larvor, Betty Van Gelderen, Olga Haenen, Eva Jansson, Eva Säker, Inger Dalsgaard, Laboratoire de Ploufragan-Plouzané-Niort [ANSES], Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), and Laboratoire de Fougères - ANSES
- Subjects
Vibrio anguillarum ,susceptibility test ,Epidemiology ,disque ,Vibrio vulnificus ,Aquaculture ,Pathogenic vibrio ,agar disc diffusion ,poisson ,MIC ,bacteria ,bactérie ,0303 health sciences ,biology ,diffusion ,04 agricultural and veterinary sciences ,Listonella anguillarum ,CLSI susceptibility testing ,microbiologic test ,%22">Fish ,contaminant ,Bioinformatica & Diermodellen ,test microbiologique ,Antimicrobial susceptibility ,[SDV.SA.ZOO]Life Sciences [q-bio]/Agricultural sciences/Zootechny ,CLSI susccptibility testing ,Aquatic Science ,minimum inhibitory concentration ,Disc diffusion ,Microbiology ,Incubation period ,Incubation time ,03 medical and health sciences ,Bio-informatics & Animal models ,pathogène ,Epidemiology, Bio-informatics & Animal models ,microbiologie ,030304 developmental biology ,agar ,Epidemiologie ,fish ,[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health ,Pathogen ,microbiology ,biology.organism_classification ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Epidemiologie, Bioinformatica & Diermodellen ,040102 fisheries ,0401 agriculture, forestry, and fisheries - Abstract
A multi-laboratory study was performed to investigate the most suitable incubation time for susceptibility tests of fish pathogens Vibrio anguillarum and Vibrio vulnificus performed at 28 degrees C. An isolate set consisting of 30 V. anguillarum and 26 V. vulnificus was used by four participating laboratories in Denmark, France, Sweden, and the Netherlands. Inhibition zone diameters were determined by agar disc diffusion for eight agents and Minimum Inhibitory Concentration (MIC) values were determined for seven agents using the standard CLSI testing protocols for non-fastidious organisms that specify 24-28 h incubation. In this work an additional set of readings was made after 48 h incubation. In total, 1120 paired zone sizes and 399 paired MIC observations were made at the two incubation times. Examination of the data demonstrated that incubation time had a small but statistically significant effect on the numerical values of susceptibility measures. However, the effects of incubation time on the precision of the data sets and the categorisation of isolates based on the application of epidemiological cutoff values were slight and statistically non significant. These analyses suggest that the susceptibility of these Vibrio species could be established using protocols that specify either 24-28 h or 44-48 h incubation.This study does not provide evidence that prolonged incubation to 48 h improves the quality of data generated by the tests. Therefore, it is recommended that the existing standard CLSI protocols with 24-28 h at 28 degrees C should be adopted for susceptibility testing of V. anguillarum and V. vulnificus. This study was performed in the frame of CoVetLab partner Insitutes https://www.covetlab.org/c5/ peer-reviewed
- Published
- 2020
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