1. Programmed cell death‐1 3′‐untranslated region polymorphism is associated with spontaneous clearance of hepatitis B virus infection
- Author
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Hajar Chihab, Fatima‐Zahra Jadid, Pelagia Foka, Imane Zaidane, Raouia El Fihry, Urania Georgopoulou, Agnes Marchio, Abdellah Elhabazi, Mohammed Chair, Pascal Pineau, Sayeh Ezzikouri, Soumaya Benjelloun, Laboratoire des Hépatites Virales [Casablanca, Maroc] (LHV), Institut Pasteur du Maroc, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Université Chouaib Doukkali (UCD), Molecular Virology Laboratory = Laboratoire de Virologie moléculaire [Athènes], Institut Pasteur Hellénique, Organisation Nucléaire et Oncogenèse / Nuclear Organization and Oncogenesis, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur du Maroc (Morocco), and Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)
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0301 basic medicine ,Untranslated region ,Male ,Programmed Cell Death 1 Receptor ,programmed cell death-1 ,medicine.disease_cause ,law.invention ,Immune tolerance ,MESH: Genotype ,PD-1 polymorphism ,law ,Genotype ,Medicine ,3' Untranslated Regions ,Polymerase chain reaction ,MESH: Aged ,MESH: Middle Aged ,MESH: Polymorphism, Single Nucleotide ,MESH: Genetic Predisposition to Disease ,MESH: Programmed Cell Death 1 Receptor ,MESH: 3' Untranslated Regions ,Middle Aged ,Hepatitis B ,3. Good health ,spontaneous clearance ,Morocco ,MESH: Hepatitis B virus ,Infectious Diseases ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Female ,Adult ,Hepatitis B virus ,chronic hepatitis B virus infection ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,MESH: Gene Expression Profiling ,Virology ,Humans ,Genetic Predisposition to Disease ,Aged ,Messenger RNA ,MESH: Humans ,MESH: Hepatitis B ,Three prime untranslated region ,business.industry ,Gene Expression Profiling ,MESH: Adult ,MESH: Male ,030104 developmental biology ,MESH: Morocco ,business ,MESH: Female ,CD8 - Abstract
International audience; Hepatitis B virus (HBV)-specific CD8+ T cells play an important role in the clearance of HBV infection. Programmed cell death-1 (PD-1), an immunosuppressive molecule that regulates T-cell activation and peripheral immune tolerance, is increasingly shown to influence the outcome of HBV infection. rs10204525, a single-nucleotide polymorphism in the 3'-untranslated region (3'-UTR) of PD-1, has been associated with susceptibility and disease progression of chronic HBV infection in far-eastern patients. The aim of our study was to assess the impact of rs10204525 variation on HBV infection in Moroccan patients. A total of 236 patients with chronic HBV infection and 134 individuals with spontaneous HBV resolution were genotyped using a Taqman assay. In addition, PD-1 mRNA expression in peripheral blood nuclear cells was determined by quantitative reverse-transcription polymerase chain reaction. We found that the AA genotype is protective (odds ratio, 0.43; 95% confidence interval, 0.19 to 0.97; P = 0.038) against HBV infection. Interestingly, PD-1 messenger RNA (mRNA) expression analysis has revealed that chronic HBV carriers with GG and GA displayed higher levels of PD-1 mRNA compared with corresponding genotypes in resolved subjects (P = 0.031 and 0.014, respectively). Our data suggest that Mediterranean HBV-infected patients carrying PD-1 GG and GA genotypes at rs10204525 have high PD-1 mRNA expression and may be more prone to installation of chronicity.
- Published
- 2018
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