Search

Your search keyword '"Patricia Flamant"' showing total 3 results
Start Over Author "Patricia Flamant" Publisher hal ccsd
3 results on '"Patricia Flamant"'

1. Muscle Injury Induces Postoperative Cognitive Dysfunction

Author

Grégory Jouvion, Lorna Guéniot, Fabrice Chrétien, Anne Danckaert, Olivier Langeron, Victoria Lepere, Gabriela Ferreira de Medeiros, Marine Le Dudal, Patricia Flamant, Pierre L. Goossens, Neuropathologie expérimentale - Experimental neuropathology, Institut Pasteur [Paris] (IP)-Université Paris Descartes - Paris 5 (UPD5), Direction Générale de l'Armement [Paris] (DGA), Ministère des armées, École Doctorale Bio Sorbonne Paris Cité [Paris] (ED562 - BioSPC), Université Sorbonne Paris Cité (USPC)-Université Paris Cité (UPCité), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), BioImagerie Photonique – Photonic BioImaging (UTechS PBI), Institut Pasteur [Paris] (IP), Department of Anesthesia and Critical Care [Créteil], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Henri Mondor [Créteil]-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service de Neuropathologie [Paris], Centre Hospitalier Sainte Anne [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-GHU Paris Psychiatrie et Neurosciences, CHU Trousseau [APHP], Maladies génétiques d'expression pédiatrique (U933), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), This work was supported by the Fondation des Gueules Cassées. Lorna Guéniot received financial support from DGA (Direction Générale de l’Armement, France) and Institut Pasteur of Paris for the realization of her PhD. The PBI-Utech is part of the France BioImaging infrastructure supported by the French National Research Agency (ANR-10-INSB-04-01, 'Investments for the future') and is grateful for the support from the Conseil de la Region Ile-de-France (program Sesame 2007, project Imagopole, S.L. Shorte)., Institut Pasteur [Paris]-Université Paris Descartes - Paris 5 (UPD5), École Doctorale Bio Sorbonne Paris Cité [Paris] (ED BioSPC), Université Sorbonne Paris Cité (USPC)-Université de Paris (UP), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Pasteur [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Maladies génétiques d'expression pédiatrique [CHU Trousseau] (Inserm U933), UF de Génétique chromosomique [CHU Trousseau], and Bodescot, Myriam

Subjects
Male, Aging, [SDV.MHEP.CHI] Life Sciences [q-bio]/Human health and pathology/Surgery, [SDV]Life Sciences [q-bio], Neuroimmunology, Hippocampus, lcsh:Medicine, Bioinformatics, Mice, [SCCO]Cognitive science, 0302 clinical medicine, Postoperative Complications, 030202 anesthesiology, Nerve Growth Factor, Medicine, lcsh:Science, 0303 health sciences, Multidisciplinary, Brain, [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, [SDV] Life Sciences [q-bio], medicine.anatomical_structure, Cytokines, Microglia, medicine.symptom, CX3C Chemokine Receptor 1, [SDV.MHEP.CHI]Life Sciences [q-bio]/Human health and pathology/Surgery, Article, Lesion, 03 medical and health sciences, Postoperative Cognitive Complications, Animals, Humans, Muscle, Skeletal, Neuroinflammation, 030304 developmental biology, Brain-derived neurotrophic factor, business.industry, Brain-Derived Neurotrophic Factor, lcsh:R, Skeletal muscle, [SCCO] Cognitive science, medicine.disease, Disease Models, Animal, Nerve growth factor, Synaptic plasticity, lcsh:Q, business, Postoperative cognitive dysfunction, Neurological disorders
Abstract

Postoperative cognitive dysfunction (POCD) is a major complication affecting patients of any age undergoing surgery. This syndrome impacts everyday life up to months after hospital discharge, and its pathophysiology still remains unclear. Translational research focusing on POCD is based on a wide variety of rodent models, such as the murine tibial fracture, whose severity can limit mouse locomotion and proper behavioral assessment. Besides, influence of skeletal muscle injury, a lesion encountered in a wide range of surgeries, has not been explored in POCD occurrence. We propose a physical model of muscle injury in CX3CR1GFP/+ mice (displaying green fluorescent microglial cells) to study POCD, with morphological, behavioral and molecular approaches. We highlighted: alteration of short- and long-term memory after muscle regeneration, wide microglial reactivity in the brain, including hippocampus area, 24 hours after muscle injury, and an alteration of central brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF) balance, 28 days after muscle injury. Our results suggest for the first time that muscle injury can have early as well as late impacts on the brain. Our CX3CR1GFP/+ model can also facilitate microglial investigation, more specifically their pivotal role in neuroinflammation and synaptic plasticity, in the pathophysiology of POCD.

Published
2020
Full Text
View/download PDF

2. Myf5 haploinsufficiency reveals distinct cell fate potentials for adult skeletal muscle stem cells

Author

Ana Cumano, Jean-Yves Coppée, Fabrice Chrétien, Vincent Mouly, Aurélie Jory, Elisa Negroni, Guillaume Soubigou, James P. Di Santo, Barbara Gayraud-Morel, Shahragim Tajbakhsh, Patricia Flamant, Ramkumar Sambasivan, Cellules Souches et Développement, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Histopathologie humaine et Modèles animaux, Institut Pasteur [Paris] (IP), Institut de Myologie, Université Pierre et Marie Curie - Paris 6 (UPMC)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Transcriptome et Epigénome (PF2), Immunité Innée - Innate Immunity, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Développement des Lymphocytes, We acknowledge support from the Institut Pasteur, Association Française contre le Myopathies, Agence Nationale de la Recherche, MyoRes (EU Framework 7 project EuroSysStem), Optistem and Fondation pour la Recherche Medicale., Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris], Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Association française contre les myopathies (AFM-Téléthon)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Pierre et Marie Curie - Paris 6 (UPMC), and Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
animal structures, Myoblasts, Skeletal, Haploinsufficiency, Cell fate determination, Biology, MyoD, Myogenic regulatory factors, Mice, 03 medical and health sciences, Transcriptional priming, 0302 clinical medicine, Myf5, medicine, Animals, Regeneration, Cell Lineage, Muscle, Skeletal, 10. No inequality, Molecular Biology, 030304 developmental biology, Induced stem cells, 0303 health sciences, Stem cell, PAX7 Transcription Factor, Skeletal muscle, Cell Biology, Anatomy, musculoskeletal system, Molecular biology, Pax7, Cell biology, Endothelial stem cell, Adult Stem Cells, Phenotype, medicine.anatomical_structure, embryonic structures, [SDV.IMM]Life Sciences [q-bio]/Immunology, MYF5, Myogenic Regulatory Factor 5, Heterogeneity, Satellite cell, 030217 neurology & neurosurgery, Adult stem cell, Developmental Biology
Abstract

International audience; Skeletal muscle stem cell fate in adult mice is regulated by crucial transcription factors, including the determination genes Myf5 and Myod. The precise role of Myf5 in regulating quiescent muscle stem cells has remained elusive. Here we show that most, but not all, quiescent satellite cells express Myf5 protein, but at varying levels, and that resident Myf5 heterozygous muscle stem cells are more primed for myogenic commitment compared with wild-type satellite cells. Paradoxically however, heterotypic transplantation of Myf5 heterozygous cells into regenerating muscles results in higher self-renewal capacity compared with wild-type stem cells, whereas myofibre regenerative capacity is not altered. By contrast, Pax7 haploinsufficiency does not show major modifications by transcriptome analysis. These observations provide a mechanism linking Myf5 levels to muscle stem cell heterogeneity and fate by exposing two distinct and opposing phenotypes associated with Myf5 haploinsufficiency. These findings have important implications for how stem cell fates can be modulated by crucial transcription factors while generating a pool of responsive heterogeneous cells.

Published
2012
Full Text
View/download PDF

3. Human papillomavirus: cause of epithelial lacrimal sac neoplasia?

Author

Patricia Cassonnet, Steffen Heegaard, Gérard Orth, Patricia Flamant, Jan Ulrik Prause, Nicolai Christian Sjö, Christian von Buchwald, Bodil Norrild, Eye Pathology Institute, Eye Pathology Institute-University of Copenhagen = Københavns Universitet (KU), Department of Otorhinolaryngology, Rigshospitalet [Copenhagen], Copenhagen University Hospital-Copenhagen University Hospital, Papillomavirus, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institute of Medical Cell Biology and Genetics, Institute of Medical Cell Biology and Genetics-University of Copenhagen = Københavns Universitet (KU), University of Copenhagen = Københavns Universitet (KU)-Institute of Medical Cell Biology and Genetics, Eye Pathology Institute-University of Copenhagen = Københavns Universitet (UCPH), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Institute of Medical Cell Biology and Genetics-University of Copenhagen = Københavns Universitet (UCPH)

Subjects
Male, MESH: Human papillomavirus 11, MESH: Carcinoma, Pathology, MESH: DNA Replication, MESH: Human papillomavirus 6, MESH: Papillomavirus Infections, MESH: Eye Neoplasms, Polymerase Chain Reaction, law.invention, 0302 clinical medicine, MESH: Aged, 80 and over, MESH: Autoradiography, law, In Situ Hybridization, Polymerase chain reaction, Aged, 80 and over, MESH: Aged, MESH: Middle Aged, Lacrimal Apparatus Diseases, Human papillomavirus 11, HPV infection, virus diseases, MESH: Carcinoma, Squamous Cell, Middle Aged, Lacrimal sac, female genital diseases and pregnancy complications, 3. Good health, medicine.anatomical_structure, 030220 oncology & carcinogenesis, MESH: RNA, Viral, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Carcinoma, Squamous Cell, RNA, Viral, Female, Adult, DNA Replication, medicine.medical_specialty, In situ hybridization, MESH: Lacrimal Apparatus Diseases, Biology, 03 medical and health sciences, MESH: In Situ Hybridization, medicine, Carcinoma, Humans, Aged, MESH: Carcinoma, Transitional Cell, Carcinoma, Transitional Cell, MESH: Humans, Papilloma, Eye Neoplasms, Papillomavirus Infections, MESH: Adult, MESH: Polymerase Chain Reaction, Transitional epithelium, Human papillomavirus 6, medicine.disease, Dacryocystitis, MESH: Male, MESH: DNA, Viral, Ophthalmology, DNA, Viral, 030221 ophthalmology & optometry, Autoradiography, MESH: Papilloma, MESH: Female
Abstract

Purpose: Epithelial tumours of the lacrimal sac are rare but important entities that may carry grave prognoses. In this study the prevalence and possible role of human papillomavirus (HPV) infection in epithelial tumours of the lacrimal sac were evaluated. Methods: Five papillomas and six carcinomas of the lacrimal sac were investigated for the presence of HPV using the polymerase chain reaction (PCR) technique. Fifteen specimens of dacryocystitis were included in the PCR reactions as controls. Furthermore, DNA in situ hybridization (ISH) and RNA ISH were performed. Results: Low-risk HPV types 6 or 11 were identified in all four lacrimal sac papillomas suitable for PCR analysis and in situ hybridization. Four of six lacrimal sac carcinomas harboured HPV. One carcinoma was positive for HPV 11 only, two carcinomas had concomitant infection with HPV 6 or 11 and high-risk HPV 16, and the remaining carcinoma was positive for HPV 16. All specimens of dacryocystitis were betaglobin-positive and HPV-negative. Using DNA ISH, two papillomas and a single carcinoma showed evidence for vegetative HPV 11 DNA replication, whereas no HPV 16 DNA replication was found in the five carcinomas tested. HPV 11 RNA was demonstrated in two papillomas. Conclusions: By analysing 11 epithelial lacrimal sac papillomas and carcinomas using PCR, DNA ISH and RNA ISH, we found HPV DNA in all investigated transitional epithelium tumours of the lacrimal sac. HPV RNA was present in two of eight epithelial lacrimal sac tumours positive for HPV DNA. As RNA degrades fast in paraffin-embedded tissue, only a small fraction of DNA-positive tumours can be expected to be RNA-positive. We therefore suggest that HPV infection is associated with the development of lacrimal sac papillomas and carcinomas.

Published
2007
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results