Search

Your search keyword '"Pierre Blandin"' showing total 7 results
Start Over Author "Pierre Blandin" Publisher hal ccsd
7 results on '"Pierre Blandin"'

1. Selective plane illumination microscope dedicated to volumetric imaging in microfluidic chambers

Author

Caroline Bissardon, Xavier Mermet, Clément Quintard, Federico Sanjuan, Yves Fouillet, Frédéric Bottausci, Marie Carriere, Florence Rivera, Pierre Blandin, Département Microtechnologies pour la Biologie et la Santé (DTBS), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire des Fluides Complexes et leurs Réservoirs (LFCR), TOTAL FINA ELF-Université de Pau et des Pays de l'Adour (UPPA)-Centre National de la Recherche Scientifique (CNRS), Chimie Interface Biologie pour l’Environnement, la Santé et la Toxicologie (CIBEST ), SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé (SYMMES), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)

Subjects
[PHYS]Physics [physics], [SDV]Life Sciences [q-bio], Atomic and Molecular Physics, and Optics, Article, Biotechnology
Abstract

International audience; In this article, we are presenting an original selective plane illumination fluorescence microscope dedicated to image “Organ-on-chip”-like biostructures in microfluidic chips. In order to be able to morphologically analyze volumetric samples in development at the cellular scale inside microfluidic chambers, the setup presents a compromise between relatively large field of view (∼ 200 µm) and moderate resolution (∼ 5 µm). The microscope is based on a simple design, built around the chip and its microfluidic environment to allow 3D imaging inside the chip. In particular, the sample remains horizontally avoiding to disturb the fluidics phenomena. The experimental setup, its optical characterization and the first volumetric images are reported.

Published
2022
Full Text
View/download PDF

2. Phase and fluorescence imaging with a surprisingly simple microscope based on chromatic aberration

Author

Ondřej Mandula, Jean-Philippe Kleman, Cédric Allier, Françoise Lacroix, Sophie Morales, Pierre Blandin, Daniel Fiole, Dorothee C. Kraemer, Lionel Hervé, Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de biologie structurale (IBS - UMR 5075), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Plateforme M4D, Cross-Disciplinary Program on Instrumentation and Detection of CEA, and French Alternative Energies and Atomic Energy Commission

Subjects
Fluorescence-lifetime imaging microscopy, Microscope, Materials science, Optical Phenomena, Image quality, Phase (waves), Image processing, 02 engineering and technology, Hippocampus, 01 natural sciences, law.invention, 010309 optics, Optics, law, 0103 physical sciences, Chromatic aberration, Animals, Humans, Image resolution, Neurons, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], business.industry, Optical Imaging, 021001 nanoscience & nanotechnology, Atomic and Molecular Physics, and Optics, Micrococcus luteus, Cardinal point, Microscopy, Fluorescence, 0210 nano-technology, business, HeLa Cells
Abstract

We propose a simple and compact microscope combining phase imaging with multi-color fluorescence using a standard bright-field objective. The phase image of the sample is reconstructed from a single, approximately 100 μm out-of-focus image taken under semi-coherent illumination, while fluorescence is recorded in-focus in epi-fluorescence geometry. The reproducible changes of the focus are achieved with specifically introduced chromatic aberration in the imaging system. This allows us to move the focal plane simply by changing the imaging wavelength. No mechanical movement of neither sample nor objective or any other part of the setup is therefore required to alternate between the imaging modality. Due to its small size and the absence of motorized components the microscope can easily be used inside a standard biological incubator and allows long-term imaging of cell culture in physiological conditions. A field-of-view of 1.2 mm2 allows simultaneous observation of thousands of cells with micro-meter spatial resolution in phase and multi-channel fluorescence mode. In this manuscript we characterize the system and show a time-lapse of cell culture in phase and multi-channel fluorescence recorded inside an incubator. We believe that the small dimensions, easy usage and low cost of the system make it a useful tool for biological research.

Published
2020
Full Text
View/download PDF

3. Statistical study of blood cell populations by very wide-field bimodal phase/ fluorescence imaging

Author

Isaure Le Cardinal de Kernier, Sophie Morales, Pierre Blandin, Anaïs Ali-Cherif, Stéphanie Bressieux, Nelly Rongeat, Serge Monneret, Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut FRESNEL (FRESNEL), Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU), HORIBA Medical (HORIBA ABX SAS), HORIBA Scientific [France], MOSAIC (MOSAIC), Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU), Laboratoire d'Electronique et des Technologies de l'Information (CEA-LETI), Université Grenoble Alpes (UGA)-Direction de Recherche Technologique (CEA) (DRT (CEA)), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), and Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Multimodal imaging, 0303 health sciences, Fluorescence-lifetime imaging microscopy, [PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics], Materials science, medicine.diagnostic_test, Resolution (electron density), Phase (waves), Wide field, optics, Flow cytometry, Blood cell, 03 medical and health sciences, 0302 clinical medicine, medicine.anatomical_structure, statistics, 030220 oncology & carcinogenesis, Microscopy, medicine, microscopy, fluorescence, phase, in-line holography, 030304 developmental biology, Biomedical engineering
Abstract

Very wide-field of view imaging can provide statistical data on large cell populations in a single acquisition. In this paper, we describe a multimodal imaging system combining brightfield, phase and fluorescence contrasts. Its greater simplicity and lower cost compared to flow cytometry make it suitable for Point-Of-Care applications. The system’s resolution was characterized on calibrated beads and resolution targets. We illustrate the potential of the single-shot imaging approach in hematology by studying the specific morphologies of white blood cell sub-types. The results suggest that very wide field of view imaging could be an alternative to flow cytometry for some applications in hematology.

Published
2019
Full Text
View/download PDF

4. Large field-of-view phase and fluorescence mesoscope with microscopic resolution

Author

Pierre Blandin, Serge Monneret, Olivier Cioni, Isaure Le Cardinal de Kernier, Julien Savatier, Nelly Rongeat, Sophie Morales, Anaïs Ali-Cherif, Institut FRESNEL (FRESNEL), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), HORIBA Medical (HORIBA ABX SAS), HORIBA Scientific [France], and Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU)

Subjects
Paper, Cytological Techniques, Biomedical Engineering, Holography, Phase (waves), 01 natural sciences, in-line holography Paper 180667R, law.invention, 010309 optics, Biomaterials, [SPI]Engineering Sciences [physics], law, Cell Line, Tumor, 0103 physical sciences, Microscopy, Image Processing, Computer-Assisted, Humans, phase, Image resolution, Large field of view, High magnification, Resolution (electron density), Equipment Design, Fluorescence, Atomic and Molecular Physics, and Optics, Electronic, Optical and Magnetic Materials, Microscopy, Fluorescence, microscopy, fluorescence, Biological system, in-line holography, Algorithms
Abstract

International audience; Phase and fluorescence are complementary contrasts that are commonly used in biology. However, the coupling of these two modalities is traditionally limited to high magnification and complex imaging systems. For statistical studies of biological populations, a large field-of-view is required. We describe a 30 mm(2) field-of-view dual-modality mesoscope with a 4-mu m resolution. The potential of the system to address biological questions is illustrated on white blood cell numeration in whole blood and multiwavelength imaging of the human osteosarcoma (U2-OS) cells.

Published
2019
Full Text
View/download PDF

5. Simultaneous multiscale and bimodal imaging using lensfree microscopy

Author

Jean-Marc Dinten, Isaure Le Cardinal de Kernier, Matthieu Leroy, Pierre Blandin, Serge Monneret, Cédric Allier, Thomas Bordy, Ondrej Mandula, CEA-LETI - Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information, Institut FRESNEL (FRESNEL), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), MOSAIC (MOSAIC), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU), and Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU)

Subjects
Materials science, business.industry, media_common.quotation_subject, Resolution (electron density), Holography, 01 natural sciences, law.invention, 010309 optics, Optics, law, 0103 physical sciences, Microscopy, Fluorescence microscope, [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, Contrast (vision), Digital holographic microscopy, 010306 general physics, business, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, Digital holography, ComputingMilieux_MISCELLANEOUS, media_common
Abstract

Lensfree in-line holography permits to image large fields of view with resolution of few micrometers. By adding a fluorescence microscopy detection pathway, we can simultaneously image samples with a better resolution and a complementary contrast.

Published
2017
Full Text
View/download PDF

6. Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source

Author

Zsolt Lenkei, Frédéric Druon, Patrick Georges, Sandrine Lévêque-Fort, Marie-Claude Potier, Pierre Blandin, Sandrine Lécart, Jack-Christophe Cossec, Laboratoire de Photophysique Moléculaire (PPM), Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11), Laboratoire Charles Fabry de l'Institut d'Optique / Elsa, Laboratoire Charles Fabry de l'Institut d'Optique (LCFIO), Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Paris-Sud - Paris 11 (UP11), Neurobiologie et diversité cellulaire (NDC), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Fluorescence-lifetime imaging microscopy, Optics and Photonics, Materials science, Time Factors, Materials Science (miscellaneous), Green Fluorescent Proteins, Fluorescence correlation spectroscopy, 01 natural sciences, Industrial and Manufacturing Engineering, Fluorescence, Cell Line, 010309 optics, 03 medical and health sciences, Optics, Neurobiology, 0103 physical sciences, Microscopy, Protein Interaction Mapping, Fluorescence Resonance Energy Transfer, Humans, Photoactivated localization microscopy, Business and International Management, 030304 developmental biology, Fluorescent Dyes, 0303 health sciences, [PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics], Total internal reflection fluorescence microscope, Super-resolution microscopy, business.industry, Lasers, Reproducibility of Results, Equipment Design, Supercontinuum, Refractometry, Microscopy, Fluorescence, Light sheet fluorescence microscopy, business
Abstract

International audience; We present the instrumental development of a versatile total internal reflection fluorescence lifetime imaging microscopy setup illuminated by a supercontinuum laser source. It enables performing wide- field fluorescence lifetime imaging with subwavelength axial resolution for a large range of fluorophores. The short overall acquisition time and the axial resolution are well suited for dynamic neurobiological applications.

Published
2009

7. Diode-pumped passively mode-locked Nd:YVO4 laser at 914 nm

Author

François Balembois, Patrick Georges, Marie-Pierre Fontaine-Aupart, Sandrine Lévêque-Fort, F. Druon, Pierre Blandin, Laboratoire Charles Fabry de l'Institut d'Optique / Elsa, Laboratoire Charles Fabry de l'Institut d'Optique (LCFIO), Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Paris-Sud - Paris 11 (UP11), Laboratoire de Photophysique Moléculaire (PPM), and Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)

Subjects
[PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics], Materials science, business.industry, Optical transition, chemistry.chemical_element, Semiconductor saturable absorber, Laser, 01 natural sciences, Neodymium, Atomic and Molecular Physics, and Optics, law.invention, Ion, 010309 optics, Optics, Mode-locking, chemistry, law, 0103 physical sciences, 010306 general physics, business, Ultrashort pulse, Diode
Abstract

International audience; We demonstrate, for the first time, to our knowledge, a diode-pumped passively mode-locked Nd:YVO4 laser, operating on the 4F3/2-4I9/2 transition of the neodymium ion at 914 nm. We obtained 8.8 ps pulses at approximately 914 nm at a repetition rate of 94 MHz, and an averaged output power of 87 mW by using a semiconductor saturable absorber mirror

Published
2006

Catalog

Books, media, physical & digital resources
See catalog results