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5 results on '"Quintana, Mercedes"'

1. Efficacy of Targeted Radionuclide Therapy Using [131I]ICF01012 in 3D Pigmented BRAF- and NRAS-Mutant Melanoma Models and In Vivo NRAS-Mutant Melanoma

Author

Akil, Hussein, Quintana, Mercedes, Raymond, Jérémy, Billoux, Tommy, Benboubker, Valentin, Besse, Sophie, Auzeloux, Philippe, Delmas, Véronique, Petit, Valérie, Larue, Lionel, D’Incan, Michel, Degoul, Françoise, Rouanet, Jacques, Imagerie Moléculaire et Stratégies Théranostiques (IMoST), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA), Contrôle de la Réponse Immune B et des Lymphoproliférations (CRIBL), Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Jean Perrin [Clermont-Ferrand] (UNICANCER/CJP), UNICANCER, CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand, Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM), Signalisation, radiobiologie et cancer, Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), CHU Estaing [Clermont-Ferrand], and delmas, veronique

Subjects
[SDV] Life Sciences [q-bio], melanoma spheroid model, NRAS mutation, BRAF mutation, [SDV]Life Sciences [q-bio], targeted radionuclide therapy, MEK inhibitors
Abstract

International audience; Purpose: To assess the efficiency of targeted radionuclide therapy (TRT), alone or in combination with MEK inhibitors (MEKi), in melanomas harboring constitutive MAPK/ERK activation responsible for tumor radioresistance. Methods: For TRT, we used a melanin radiotracer ([131I]ICF01012) currently in phase 1 clinical trial (NCT03784625). TRT alone or combined with MEKi was evaluated in three-dimensional melanoma spheroid models of human BRAFV600E SK-MEL-3, murine NRASQ61K 1007, and WT B16F10 melanomas. TRT in vivo biodistribution, dosimetry, efficiency, and molecular mechanisms were studied using the C57BL/6J-NRASQ61K 1007 syngeneic model. Results: TRT cooperated with MEKi to increase apoptosis in both BRAF- and NRAS-mutant spheroids. NRASQ61K spheroids were highly radiosensitive towards [131I]ICF01012-TRT. In mice bearing NRASQ61K 1007 melanoma, [131I]ICF01012 induced a significant extended survival (92 vs. 44 days, p < 0.0001), associated with a 93-Gy tumor deposit, and reduced lymph-node metastases. Comparative transcriptomic analyses confirmed a decrease in mitosis, proliferation, and metastasis signatures in TRT-treated vs. control tumors and suggest that TRT acts through an increase in oxidation and inflammation and P53 activation. Conclusion: Our data suggest that [131I]ICF01012-TRT and MEKi combination could be of benefit for advanced pigmented BRAF-mutant melanoma care and that [131I]ICF01012 alone could constitute a new potential NRAS-mutant melanoma treatment.

Published
2021
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2. PhytoMetaboBank, une nouvelle base de données sur les bioactifs végétaux et leurs métabolites chez l’homme

Author

Giacomoni, Franck, Rothwell, Joseph, Fillâtre, Johann, Knox, C., Cesaire, Daniel, Quintana, Mercedes, Lyan, Bernard, Sébédio, Jean-Louis, Comte, Blandine, Pujos-Guillot, Estelle, Manach, Claudine, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, In Siliflo Inc, Centre de Recherche en Nutrition Humaine (CRNH). FRA., and ProdInra, Migration

Subjects
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

National audience; The “food metabolome” comprises all metabolites present in biological fluids that are directly derived from the digestion of food. A large proportion of the food metabolome consists of phytochemical metabolites, which are products of intestinal,hepatic or microbial metabolism of plant secondary metabolites such as polyphenols, terpenoids, alkaloids...Identification of unknowns in metabolome fingerprints is a laborious step-by -step process and often a bottleneck in biomarker discovery. One major limitation for the interpretation of the Food metabolome fingerprints is the incompleteness of existing databases regarding phytochemical metabolites. As part of the ANR PhenoMeNep project, we aim to construct a new database tailored to the study of the phytochemical component of the food metabolome. Provisionally named PhytoMetaboBank, the data base will be an inventory of known metabolites described in the literature for all dietary phytochemicals. It will also include the most likely metabolites predicted in silico for these dietary phytochemicals. Built with MySQL and Perl processing chains, an efficient elational design will underpin a powerful and intuitive web interface. For a queried monoisotopic mass or elemental formula, the database will return a list of possible metabolites, with their physicochemical properties, spectral data and possible dietary precursors linked to food sources. PhytoMetaboBank will be the first database to collate information on phytochemical metabolites from a metabolomics standpoint, and should improve the identification of discriminant ions in non-targeted profiling

Published
2016

3. PhytoHUB: a new database dedicated to dietary phytochemicals and their human metabolites for nutritional metabolomics

Author

Giacomoni, Franck, Fillatre, Yann, Rothwell, J.A, Knox, Craig, Eisner, Roman, Cesaire, Daniel, Quintana, Mercedes, Comte, Blandine, Pujos-Guillot, Estelle, Manach, Claudine, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, In Siliflo Inc, ANR Phenomenep ALIA-2010-007 / Conseil Regional Auvergne-FEDER post-doc grant (YF), and Metabolomics Society.

Subjects
alcaloide, base de données, polyphénol, terpénoïde, phytochimie, Alimentation et Nutrition, Food and Nutrition, métabolisme digestif, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, ComputingMilieux_MISCELLANEOUS
Abstract

Présentation Claudine Manach; International audience

Published
2013

4. Fibrinolytic activity is associated with presence of cystic media degeneration in aneurysms of the ascending aorta

Author

Borges, Luciano F, Gomez, Delphine, Quintana, Mercedes, Leclercq, Anne, Touat, Ziad, Jondeau, Guillaume, Meilhac, Olivier, Jandrot-Perrus, Martine, Gutierrez, Paulo Sampaio, Freymuller, Edna, Vranckx, Roger, Michel, Jean-Baptiste, Electron Microscopy Center, University Federal of Sao Paulo, Hémostase, bio-ingénierie et remodelage cardiovasculaires (LBPC), Université Paris Diderot - Paris 7 (UPD7)-Université Paris 13 (UP13)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Galilée, Centre de référence MARFAN, Hôpital Bichat, Laboratory of Pathology, and INCOR

Subjects
parasitic diseases, cardiovascular system, Medicine, complex mixtures, digestive system diseases
Abstract

International audience; Aims Thoracic Ascending Aortic Aneurysms (TAA) are characterized by elastic fibre breakdown and cystic medial degeneration within the aortic media, associated with progressive SMC rarefaction. The TGF-β/Smad2 signaling pathway is involved in this process. Since pericellular fibrinolytic system activation is able to degrade adhesive proteins, activate MMPs, induce SMC disappearance and increase the bio-availability of TGF-β, we explored the plasminergic system in TAA. Methods and Results Ascending aortas (21 controls and 19 TAAs (of 3 different aetiologies)) were analyzed. Immunohistochemistry showed accumulation of t-PA, u-PA & plasmin in TAAs, associated with remaining SMCs. Over-expression of t-PA, and u-PA was confirmed by RT-PCR, immunoblotting and zymography on TAA extracts and culture medium conditioned by TAA. Plasminogen was present on the SMC surface and inside cytoplasmic vesicles, but plasminogen mRNA was undetectable in TAA medial layer. Plasmin-antiplasmin complexes were detected in TAA-conditioned medium and activation of the fibrinolytic system was associated with an increased fibronectin turnover. Fibronectin-related material was detected immunohistochamically in dense clumps around SMCs and co-localized with Latent TGF-β Binding Protein-1. Conclusions These observations indicate that the fibrinolytic pathway could play a critical role in TAA progression, via direct or indirect impact on ECM and consecutive modulation of TGF-β bio-availability.

Published
2010
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5. Inter-individual variability of protein patterns in saliva of healthy adults

Author

Patrick Ducoroy, Géraldine Lucchi, Christian Salles, Christophe Chambon, Mercedes Quintana, Olivier Palicki, Martine Morzel, Imagerie Moléculaire et Stratégies Théranostiques (IMoST), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Centre des Sciences du Goût (CSG), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Plate-forme Protéomique CLIPP - Clinical and Innovation Proteomic Platform [Dijon] (CLIPP), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] (ICMUB), Université de Bourgogne (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Qualité des produits animaux-Plateforme Proteomique, Institut National de la Recherche Agronomique (INRA), Hydrosciences Montpellier (HSM), Institut de Recherche pour le Développement (IRD)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] (ICMUB), Institut national des sciences de l'Univers (INSU - CNRS)-Institut de Recherche pour le Développement (IRD)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Quintana, Mercedes, Imagerie Moléculaire et Stratégies Théranostiques - Clermont Auvergne (IMoST), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Université de Franche-Comté (UFC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Technologie de Belfort-Montbeliard (UTBM)-Université de Franche-Comté (UFC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Technologie de Belfort-Montbeliard (UTBM)-Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] (ICMUB), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), and Institut de Recherche pour le Développement (IRD)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Adult, Male, Proteomics, Saliva, [CHIM.ANAL] Chemical Sciences/Analytical chemistry, Proteome, Biophysics, Biochemistry, Mass Spectrometry, SALIVA, 03 medical and health sciences, 0302 clinical medicine, Immune system, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Humans, Electrophoresis, Gel, Two-Dimensional, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Amylase, Salivary Proteins and Peptides, protéome, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, chemistry.chemical_classification, Gel electrophoresis, Analysis of Variance, 0303 health sciences, biology, Gene Expression Profiling, Biodiversity, 030206 dentistry, Middle Aged, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences, Molecular biology, [SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciences, Enzyme, chemistry, SALIVARY PATTERNS, Transferrin, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Multivariate Analysis, biology.protein, Female, Analysis of variance, INTER-INDIVIDUAL VARIABILITY
Abstract

International audience; In order to document inter-individual variability in salivary protein patterns, unstimulated whole saliva was obtained from 12 subjects at 10 am and 3 pm of the same day. Saliva proteins were separated using two-dimensional gel electrophoresis, and semi-quantified using image analysis. One-way ANOVA was used to test the effects “time of sampling” and “subject”. Data were further explored by multivariate analyses (PCA, hierarchical clustering). Spots of interest were identified by mass spectrometry (MALDI-TOF MS/MS and nanoLC ESI-IT MS/MS). A dataset of 509 spots matched in all gels was obtained. There was no diurnal statistical effect on salivary patterns while inter-individual variability was high with 47 spots differentially expressed between subjects (p < 1%). Clustering of these spots revealed that subjects could be discriminated first based on several proteins participating to the non-specific immune response (cystatins, lipocalin 1, parotid-secretory protein and prolactin-induced protein). Independently, subjects were also differentiated by their level of proteins originating from serum and involved in the immune system (complement C3, transferrin, IgG2), as well as the relative abundance of enzymes involved in carbohydrates metabolism (amylase and glycolytic enzymes). Inter-individual variability should be accounted for when searching for salivary biomarkers or when studying in-mouth biochemical mechanisms.

Published
2009
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