1. Developmental expression of the Notch signaling pathway genes during mouse preimplantation development
- Author
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Sarah Cormier, Michel Cohen-Tannoudji, Sandrine Vandormael-Pournin, Charles Babinet, Biologie du Développement, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), We thank Corinne Chureau-Pommier for providing TS cell total RNAs. This work was funded by the Centre National de la Recherche Scientifique and the Pasteur Institute (GPH 07 Program). Sarah Cormier is recipient of a fellowship from Association de la Recherche contre le Cancer., and Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)
- Subjects
MESH: Signal Transduction ,Time Factors ,MESH: Trophoblasts ,Trophoblast stem cells ,MESH: Calcium-Binding Proteins ,Polymerase Chain Reaction ,Mice ,0302 clinical medicine ,MESH: Reverse Transcriptase Polymerase Chain Reaction ,MESH: Gene Expression Regulation, Developmental ,Inner cell mass ,Serrate-Jagged Proteins ,MESH: Animals ,[SDV.BDD]Life Sciences [q-bio]/Development Biology ,Genetics ,0303 health sciences ,Receptors, Notch ,Reverse Transcriptase Polymerase Chain Reaction ,Stem Cells ,Intracellular Signaling Peptides and Proteins ,Gene Expression Regulation, Developmental ,Nuclear Proteins ,Early mouse development ,Trophoblasts ,Cell biology ,DNA-Binding Proteins ,Rbpsuh ,Phenotype ,medicine.anatomical_structure ,Immunoglobulin J Recombination Signal Sequence-Binding Protein ,embryonic structures ,Intercellular Signaling Peptides and Proteins ,Jagged ,MESH: Membrane Proteins ,Signal Transduction ,JAG2 ,MESH: DNA Primers ,JAG1 ,Embryonic stem cells ,MESH: Jagged-1 Protein ,Ubiquitin-Protein Ligases ,Notch signaling pathway ,Deltex ,MESH: Stem Cells ,Cell fate determination ,Biology ,MESH: Phenotype ,03 medical and health sciences ,MESH: Intracellular Signaling Peptides and Proteins ,medicine ,MESH: Serrate-Jagged Proteins ,Animals ,Cell Lineage ,RNA, Messenger ,Blastocyst ,MESH: Intercellular Signaling Peptides and Proteins ,Molecular Biology ,MESH: Mice ,DNA Primers ,030304 developmental biology ,MESH: RNA, Messenger ,Calcium-Binding Proteins ,MESH: Time Factors ,Membrane Proteins ,Trophoblast ,MESH: Polymerase Chain Reaction ,MESH: Immunoglobulin J Recombination Signal Sequence-Binding Protein ,MESH: Cell Lineage ,Embryonic stem cell ,MESH: Blastocyst ,Notch receptors ,Delta-like ,MESH: Receptors, Notch ,MESH: Nuclear Proteins ,Jagged-1 Protein ,030217 neurology & neurosurgery ,MESH: DNA-Binding Proteins ,Developmental Biology - Abstract
3. Note added in proofAfter the present paper was submitted, two whole genome studies on gene expression dynamics during mouse preimplantation development using microarrays were reported (Hamatani et al., and Wang et al., Developmental Cell, (2004), Vol.6, pp. 117–131 and pp. 133–144, respectively). Expression of multiple components of the Notch signaling pathway was demonstrated including three of the genes (Notch3, Dtx2 and Jag2) examined in our study; International audience; Notch signaling is an evolutionary conserved pathway involved in intercellular signaling and essential for proper cell fate choices during development. Thus, it could be involved in mouse preimplantation development where intercellular signaling plays a crucial role, particularly between the inner cell mass and the trophectoderm of the blastocyst. At their face value, the phenotypes observed when disrupting each of the four Notch genes known in the mouse do not support this view as none of them involves perturbation of preimplantation development. However this could be due to functional redundancy and/or maternal expression. As a first step to address this issue, we decided to examine the expression in early development of various genes known to participate in Notch signaling. Here, we report on the expression pattern of Notch1-4, Jagged1 (Jag1), Jag2, Delta-like1 (Dll-1), Dll-3, Dll-4, Rbpsuh, Deltex1(Dtx1)and Dtx2 genes during preimplantation development from unfertilized eggs until late blastocyst stage using a RT-PCR strategy. We show that Notch1, 2, Jag1-2, Dll-3, Rbpsuh and Dtx2 transcripts are expressed at all stages. Notch4 and Dll-4 mRNAs are synthesized from the 2-cell through to the hatched blastocyst stage. Notch3, Dll-1 and Dtx1exhibit a stage dependent expression as their mRNAs are detected in 2-cell embryos and in hatched blastocysts, but are absent or weakly detected at the morula stage. Finally, we show that all the above genes are expressed both in Embryonic and Trophoblast Stem cells (ES and TS cells, respectively). Our results suggest that the Notch pathway may be active during mouse preimplantation development.
- Published
- 2004